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Protoplast Transformation of Filamentous Fungi

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Molecular and Cell Biology Methods for Fungi

Part of the book series: Methods in Molecular Biology ((MIMB,volume 638))

Abstract

The protoplast method for the transformation of filamentous fungi is described in detail, as is the Restriction Enzyme-Mediated Integration (REMI) procedure for introducing tagged mutations into the fungal genome. A split marker method for generating PCR fragments for targeted integration and deletion of genes of interest is also detailed.

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Acknowledgments

We thank previous lab members (Shun-Wen Lu, Natalie Catlett) who contributed to developing transformation, REMI, and split marker protocols.

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Authors and Affiliations

  1. Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY, USA

    B. Gillian Turgeon, Bradford Condon, Jinyuan Liu & Ning Zhang

Authors
  1. B. Gillian Turgeon
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  2. Bradford Condon
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  3. Jinyuan Liu
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  4. Ning Zhang
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Editor information

Editors and Affiliations

  1. Dept. Plant Sciences, Tel Aviv University, Tel Aviv, 69978, Israel

    Amir Sharon

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Turgeon, B.G., Condon, B., Liu, J., Zhang, N. (2010). Protoplast Transformation of Filamentous Fungi. In: Sharon, A. (eds) Molecular and Cell Biology Methods for Fungi. Methods in Molecular Biology, vol 638. Humana Press. https://doi.org/10.1007/978-1-60761-611-5_1

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  • DOI: https://doi.org/10.1007/978-1-60761-611-5_1

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-610-8

  • Online ISBN: 978-1-60761-611-5

  • eBook Packages: Springer Protocols

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