Abstract
The use of small interfering RNAs (siRNAs) in human therapy may be hindered by the recruitment of nonspecific effects such as the activation of innate immune responses. Recently, several innate immune receptors have been implicated in the detection of siRNAs. This chapter provides a brief overview of the current knowledge of siRNA-induced innate immunity, as well as protocols for the rapid identification of siRNAs with innate immune stimulatory activity.
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Acknowledgments
This work was supported by NIH grant P01 CA062220 and NHMRC grant 491106.
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Gantier, M.P., Williams, B.R.G. (2010). Monitoring Innate Immune Recruitment by siRNAs in Mammalian Cells. In: Min, WP., Ichim, T. (eds) RNA Interference. Methods in Molecular Biology, vol 623. Humana Press. https://doi.org/10.1007/978-1-60761-588-0_2
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DOI: https://doi.org/10.1007/978-1-60761-588-0_2
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