Abstract
Protein expression is the most complex metabolic reaction that has been encapsulated in liposomes, mainly as an intermediate step toward the synthesis of minimal semisynthetic cells. Although there are different experimental approaches to achieving the synthesis of proteins inside liposomes and it is therefore not possible to give a standard recipe, all methods follow a general strategy, which is briefly discussed. On this basis, we provide general indications for designing and realizing protein-expressing liposomes. Our approach for the green fluorescent protein expression inside 200-nm extruded vesicles is described in detail.
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Acknowledgements
This work was carried out within the SYNTHCELLS project (Approaches to the Bioengineering of Synthetic Minimal Cells, EU Grant #FP6-043359) and further supported by the Human Frontiers Science Program.
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Stano, P., Kuruma, Y., de Souza, T.P., Luisi, P.L. (2010). Biosynthesis of Proteins Inside Liposomes. In: Weissig, V. (eds) Liposomes. Methods in Molecular Biology™, vol 606. Humana Press. https://doi.org/10.1007/978-1-60761-447-0_11
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DOI: https://doi.org/10.1007/978-1-60761-447-0_11
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