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Defining In Vivo Dendritic Cell Functions Using CD11c-DTR Transgenic Mice

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Dendritic Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 595))

Abstract

The study of dendritic cell involvement in complex phenomena that rely on multi-cellular interactions, such as immune homeostasis, stimulation, and tolerization, called for the investigation of dendritic cell functions within physiological context. To this end we have developed a conditional cell ablation strategy that is based on dendritic cell-restricted expression of a Diphtheria Toxin receptor (DTR) using the CD11c/Itgax promoter. Here, we provide basic protocols that describe the use of this prototypic dendritic cell ablation model and highlight pitfalls and strengths of the approach.

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Acknowledgments

We would like to thank the past and present members of the Jung lab for sharing their protocols. This work was supported by the Israel Science Foundation (grant # 1057/03). S.J. is the incumbent of the Pauline Recanati Career Development Chair.

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Authors and Affiliations

  1. Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel

    Liat Bar-On & Steffen Jung

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  1. Liat Bar-On
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  2. Steffen Jung
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Editors and Affiliations

  1. Quellijnstraat 25A, Amsterdam, 1072 XN, Netherlands

    Shalin H. Naik

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© 2010 Humana Press, a part of Springer Science+Business Media, LLC

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Bar-On, L., Jung, S. (2010). Defining In Vivo Dendritic Cell Functions Using CD11c-DTR Transgenic Mice. In: Naik, S. (eds) Dendritic Cell Protocols. Methods in Molecular Biology, vol 595. Humana Press. https://doi.org/10.1007/978-1-60761-421-0_28

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  • DOI: https://doi.org/10.1007/978-1-60761-421-0_28

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-420-3

  • Online ISBN: 978-1-60761-421-0

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