Abstract
There are many in vitro methods for evaluating antioxidant activity. In this chapter, we describe an operationally simple cell-based assay, oxidative hemolysis inhibition assay (OxHLIA). OxHLIA is based on inhibition of free radical-induced membrane damage in erythrocytes by antioxidants. The advantage of this method is that it uses peroxyl radicals as pro-oxidants and erythrocytes as oxidizable targets so that the results obtained reflect biologically relevant radical-scavenging activity and microlocalization of antioxidants. We also present here a comparison of OxHLIA with other common methods (DPPH, ABTS•+, and ORAC assays).
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Acknowledgments
This work was partly supported by a Grant-in-Aid for Young Scientists (B) (No. 17780103) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
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Takebayashi, J., Chen, J., Tai, A. (2010). A Method for Evaluation of Antioxidant Activity Based on Inhibition of Free Radical-Induced Erythrocyte Hemolysis. In: Armstrong, D. (eds) Advanced Protocols in Oxidative Stress II. Methods in Molecular Biology, vol 594. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-411-1_20
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DOI: https://doi.org/10.1007/978-1-60761-411-1_20
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