Abstract
Hypertension is a leading cause of heart attack, stroke, and kidney failure and represents a serious medical issue worldwide. The genetic basis of hypertension is well-established, but few causal genes have been identified thus far. Non-invasive blood pressure measurements are a critical component of high-throughput genetic studies to identify genes controlling blood pressure. Whereas this technique is fairly routine for blood pressure measurements in rats, non-invasive blood pressure measurement in mice has proven to be more challenging. This chapter describes an experimental protocol measuring blood pressure in mice using a CODA non-invasive blood pressure monitoring system. This method enables accurate blood pressure phenotyping in mice for linkage or mutagenesis studies, as well as for other experiments requiring high-throughput blood pressure measurement.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Chobanian, AV, Bakris, GL, Black, HR, et al. (2003) Seventh Report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure. Hypertension 42(6), 1206–1252.
Cowley, AW. (2006) The genetic dissection of essential hypertension. Nat Rev Genet 7(11), 829–840.
Manunta, P, Bianchi, G. (2006) Pharmacogenomics and pharmacogenetics of hypertension: update and perspectives – the adducin paradigm. J Am Soc Nephrol 17(4), S30–S35.
Pravenec, M, Kurtz, TW. (2007) Molecular genetics of experimental hypertension and the metabolic syndrome – from gene pathways to new therapies. Hypertension 49(5), 941–952.
Kurtz, TW, Griffin, KA, Bidani, AK, et al. (2005) Recommendations for blood pressure measurement in humans and experimental animals – Part 2: blood pressure measurement in experimental animals – A statement for professionals from the subcommittee of professional and public education of the American heart association council on high blood pressure research. Hypertension 45(2), 299–310.
Feng, MJ, Whitesall, S, Zhang, YY, et al. (2008) Validation of volume-pressure recording tail-cuff blood pressure measurements. Am J Hypertens 21(12), 1288–1291.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Feng, M., DiPetrillo, K. (2009). Non-invasive Blood Pressure Measurement in Mice. In: DiPetrillo, K. (eds) Cardiovascular Genomics. Methods in Molecular Biology™, vol 573. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-247-6_3
Download citation
DOI: https://doi.org/10.1007/978-1-60761-247-6_3
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-246-9
Online ISBN: 978-1-60761-247-6
eBook Packages: Springer Protocols