Summary
Switchgrass (Panicum virgatum L.) is a highly productive warm-season C4 species that is being developed into a dedicated biofuel crop. This chapter describes a protocol that allows the generation of transgenic switchgrass plants by Agrobacterium tumefaciens-mediated transformation. Embryogenic calluses induced from caryopses or inflorescences were used as explants for inoculation with A. tumefaciens strain EHA105. Hygromycin phosphotransferase gene (hph) was used as the selectable marker and hygromycin was used as the selection agent. Calluses resistant to hygromycin were obtained after 5–6 weeks of selection. Soil-grown switchgrass plants were regenerated about 6 months after callus induction and Agrobacterium-mediated transformation.
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References
Vogel, K.P. and Jung, H.J.G. (2001) Genetic modification of herbaceous plants for feed and fuel. Crit. Rev. Plant Sci. 20, 15–49
McLaughlin, S.B. and Kszos, L.A. (2005) Development of switchgrass (Panicum virgatum) as a bioenergy feedstock in the United States. Biomass Bioenergy 28, 515–535
Wang, Z.-Y. and Ge, Y. (2006) Recent advances in genetic transformation of forage and turf grasses. In Vitro Cell. Dev. Biol., Plant 42, 1–18
Richards, H.A., Rudas, V.A., Sun, H., McDaniel, J.K., Tomaszewski, Z. and Conger, B.V. (2001) Construction of a GFP-BAR plasmid and its use for switchgrass transformation. Plant Cell Rep. 20, 48–54
Somleva, M.N., Tomaszewski, Z. and Conger, B.V. (2002) Agrobacterium-mediated genetic transformation of switchgrass. Crop Sci. 42, 2080–2087
Hu, T., Metz, S., Chay, C., Zhou, H.P., Biest, N., Chen, G., Cheng, M., Feng, X., Radionenko, M., Lu, F. and Fry, J. (2003) Agrobacterium-mediated large-scale transformation of wheat (Triticum aestivum L.) using glyphosate selection. Plant Cell Rep. 21, 1010–1019
Dai, S., Zheng, P., Marmey, P., Zhang, S., Tian, W., Chen, S., Beachy, R.N. and Fauquet, C. (2001) Comparative analysis of transgenic rice plants obtained by Agrobacterium-mediated transformation and particle bombardment. Mol. Breed. 7, 25–33
Moore, K.J., Moser, L.E., Vogel, K.P., Waller, S.S., Johnson, B.E. and Pedersen, J.F. (1991) Describing and quantifying growth stages of perennial forage grasses. Agron. J. 83, 1073–1077
Acknowledgment
The work is supported by the USDA-DOE Biomass Initiative grant (Project No. NRCS 68–3A75–5–239) and the Samuel Roberts Noble Foundation.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Xi, Y., Ge, Y., Wang, ZY. (2009). Genetic Transformation of Switchgrass. In: Mielenz, J. (eds) Biofuels. Methods in Molecular Biology, vol 581. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-214-8_4
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DOI: https://doi.org/10.1007/978-1-60761-214-8_4
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-213-1
Online ISBN: 978-1-60761-214-8
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