Summary
The zebrafish is a favorite model organism to study tissue morphogenesis during development at a subcellular level. This largely results from the fact that zebrafish embryos are transparent and thus accessible to various imaging techniques, such as confocal and two-photon excitation (2PE) microscopy. In particular, 2PE microscopy has been shown to be useful for imaging deep cell layers within the embryo and following tissue morphogenesis over long periods. This chapter describes how to use 2PE microscopy to study morphogenetic movements during early zebrafish embryonic development, providing a general blueprint for its use in zebrafish.
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Acknowledgments
We are grateful to P. Stockinger and F. Ulrich for providing us with images obtained by 2PE and J. Topczewski for providing us with the Tg(β-actin:hras-egfp) fish line. We also thank J. Peychl for advice and assistance with 2PE microscopy and G. Junghanns, E. Lehmann, and J. Hückmann for help with the fish care. We thank F. Friedrich for help with figures and L. Rohde for critical reading of the manuscript. This work was supported by grants from the Emmy Noether Program of the Deutschen Forschungsgemeinschaft, the European Union, and the Max Planck Society to CPH.
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Carvalho, L., Heisenberg, CP. (2009). Imaging Zebrafish Embryos by Two-Photon Excitation Time-Lapse Microscopy. In: Lieschke, G., Oates, A., Kawakami, K. (eds) Zebrafish. Methods in Molecular Biology, vol 546. Humana Press. https://doi.org/10.1007/978-1-60327-977-2_17
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DOI: https://doi.org/10.1007/978-1-60327-977-2_17
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