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Horizontal Gene Transfer in Cyanobacterial Signature Genes

  • Protocol
Horizontal Gene Transfer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 532))

Abstract

Comparison of 15 phylogenetically diverse cyanobacterial genomes identified an updated list of 183 signature genes that are widely found in cyanobacteria but absent in non-cyanobacterial species. These signature genes comprise the unique portion of the core cyanobacterial phenotype, and their absence from other lineages implies that if they arose by horizontal gene transfer (HGT), it likely occurred before the last shared cyanobacterial ancestor. A remaining issue is whether or not these signature genes would be relatively immune to HGT within the cyanobacterial lineage. Phylogenetic trees for each signature gene were constructed and compared to cyanobacterial groupings based on 16S rRNA sequences, with clear incongruence considered indicative of HGT. Approximately 18% of the signature genes exhibited such anomalies, indicating that the incidence of inter-lineage HGT has been significant. A preliminary analysis of intra-lineage transfer was conducted using four Synechococcus/Prochlorococcus species. In this case, it was found that 13% of the signature genes had likely been involved in within group HGT. In order to compare this level of likely HGT to other gene types, the analysis was extended to 1380 genes shared by the four Synechococcus/Prochlorococcus species. Successful HGT events appear to be most frequent among genes involved in photosynthesis/respiration and genes of unknown function, many of which are signature genes. This is consistent with the hypothesis that genes that most directly effect competition and adaptation of similar species in neighboring niches would be most usefully transferred. Such genes may be more easily integrated into a new genomic environment due to close similarities in regulatory circuits. In summary, signature genes are not immune from HGT and in fact may be favored candidates for HGT among closely related cyanobacterial strains.

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References

  1. Jain, R., Rivera, M. C., Moore, J. E., Lake, J. A. (2002) Horizontal gene transfer in microbial genome evolution. Theor Popul Biol 61, 489–95.

    Article  PubMed  Google Scholar 

  2. Nakasugi, K., Svenson, C. J., Neilan, B. A. (2006) The competence gene, comF, from Synechocystis sp. strain PCC 6803 is involved in natural transformation, phototactic motility and piliation. Microbiology 152, 3623–31.

    Article  CAS  PubMed  Google Scholar 

  3. Golden, S. S., Sherman, L. A. (1984) Optimal conditions for genetic transformation of the cyanobacterium Anacystis nidulans R2. J Bacteriol 158, 36–42.

    CAS  PubMed  Google Scholar 

  4. Grigorieva, G., Shestakov, S. (1982) Transformation in cyanobacterium Synechocystis sp. 6803. FEMS Microbiol Lett 13, 367–70.

    Article  CAS  Google Scholar 

  5. Stevens-Jr, S. E., Porter, R. D. (1986) Heterospecific transformation among cyanobacteria. J Bacteriology 167, 1074–6.

    CAS  Google Scholar 

  6. Trehan, K., Sinha, U. (1981) Genetic transfer in a nitrogen-fixing filamentous cyanobacterium. J Gen Microbiology 124, 349–52.

    Google Scholar 

  7. Iwai, M., Katoh, H., Katayama, M., Ikeuchi, M. (2004) Improved genetic transformation of the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-1. Plant Cell Physiol 45, 171–5.

    Article  CAS  PubMed  Google Scholar 

  8. Onai, K., Morishita, M., Kaneko, T., Tabata, S., Ishiura, M. (2004) Natural transformation of the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1: A simple and efficient method for gene transfer. Mol Genet Genomics 271, 50–9.

    Article  CAS  PubMed  Google Scholar 

  9. Smith, H. O., Danner, D. B., Deich, R. A. (1981) Genetic transformation. Annu Rev Biochem 50, 41–68.

    Article  CAS  PubMed  Google Scholar 

  10. Morrison, S. S., Mullineaux, C. W., Ashby, M. K. (2005) The influence of acetyl phosphate on DspA signalling in the cyanobacterium Synechocystis sp. PCC6803. BMC Microbiol 5, 47.

    Article  PubMed  Google Scholar 

  11. Notani, N. K., Joshi, V.P., Kanade, R.P. (1984) Genetic transformation in bacteria. J Biosci 6, 525–33.

    Article  CAS  Google Scholar 

  12. Okamoto, S., Ikeuchi, M., Ohmori, M. (1999) Experimental analysis of recently transposed insertion sequences in the cyanobacterium Synechocystis sp. PCC 6803. DNA Res 6, 265–73.

    Article  CAS  PubMed  Google Scholar 

  13. Ochman, H., Lawrence, J. G., Groisman, E. A. (2000) Lateral gene transfer and the nature of bacterial innovation. Nature 405, 299–304.

    Article  CAS  PubMed  Google Scholar 

  14. Muro-Pastor, A. M., Kuritz, T., Flores, E., Herrero, A., Wolk, C. P. (1994) Transfer of a genetic marker from a megaplasmid of Anabaena sp. strain PCC 7120 to a megaplasmid of a different Anabaena strain. J Bacteriol 176, 1093–8.

    CAS  PubMed  Google Scholar 

  15. Wolk, C. P., Vonshak, A., Kehoe, P., Elhai, J. (1984) Construction of shuttle vectors capable of conjugative transfer from Escherichia coli to nitrogen-fixing filamentous cyanobacteria. Proc Natl Acad Sci U S A 81, 1561–5.

    Article  CAS  PubMed  Google Scholar 

  16. Elhai, J., Wolk, C. P. (1988) Conjugal transfer of DNA to cyanobacteria. Methods Enzymol 167, 747–54.

    Article  CAS  PubMed  Google Scholar 

  17. Matteson, W. S. A. A. (2008) Freshwater and marine virioplankton: A brief overview of commonalities and differences. Freshw Biol 53, 1076–89.

    Article  Google Scholar 

  18. Mann, N. H., Clokie, M. R., Millard, A., Cook, A., Wilson, W. H., Wheatley, P. J., Letarov, A., Krisch, H. M. (2005) The genome of S-PM2, a “photosynthetic” T4-type bacteriophage that infects marine Synechococcus strains. J Bacteriol 187, 3188–200.

    Article  CAS  PubMed  Google Scholar 

  19. Lindell, D., Sullivan, M. B., Johnson, Z. I., Tolonen, A. C., Rohwer, F., Chisholm, S. W. (2004) Transfer of photosynthesis genes to and from Prochlorococcus viruses. Proc Natl Acad Sci U S A 101, 11013–8.

    Article  CAS  PubMed  Google Scholar 

  20. Sullivan, M. B., Coleman, M. L., Weigele, P., Rohwer, F., Chisholm, S. W. (2005) Three Prochlorococcus cyanophage genomes: Signature features and ecological interpretations. PLoS Biol 3, e144.

    Article  PubMed  Google Scholar 

  21. Weigele, P. R., Pope, W. H., Pedulla, M. L., Houtz, J. M., Smith, A. L., Conway, J. F., King, J., Hatfull, G. F., Lawrence, J. G., Hendrix, R. W. (2007) Genomic and structural analysis of Syn9, a cyanophage infecting marine Prochlorococcus and Synechococcus. Environ Microbiol 9, 1675–95.

    Article  CAS  PubMed  Google Scholar 

  22. Zeidner, G., Bielawski, J. P., Shmoish, M., Scanlan, D. J., Sabehi, G., Beja, O. (2005) Potential photosynthesis gene recombination between Prochlorococcus and Synechococcus via viral intermediates. Environ Microbiol 7, 1505–13.

    Article  CAS  PubMed  Google Scholar 

  23. Rivera, M. C., Jain, R., Moore, J. E., Lake, J. A. (1998) Genomic evidence for two functionally distinct gene classes. Proc Natl Acad Sci U S A 95, 6239–44.

    Article  CAS  PubMed  Google Scholar 

  24. Yap, W. H., Zhang, Z., Wang, Y. (1999) Distinct types of rRNA operons exist in the genome of the actinomycete Thermomonospora chromogena and evidence for horizontal transfer of an entire rRNA operon. J Bacteriol 181, 5201–9.

    CAS  PubMed  Google Scholar 

  25. Nakamura, Y., Itoh, T., Matsuda, H., Gojobori, T. (2004) Biased biological functions of horizontally transferred genes in prokaryotic genomes. Nat Genet 36, 760–6.

    Article  CAS  PubMed  Google Scholar 

  26. Shi, S. Y., Cai, X. H., Ding, D. F. (2005) Identification and categorization of horizontally transferred genes in prokaryotic genomes. Acta Biochim Biophys Sin (Shanghai) 37, 561–6.

    Article  CAS  Google Scholar 

  27. Zhaxybayeva, O., Gogarten, J. P., Charlebois, R. L., Doolittle, W. F., Papke, R. T. (2006) Phylogenetic analyses of cyanobacterial genomes: Quantification of horizontal gene transfer events. Genome Res 16, 1099–108.

    Article  CAS  PubMed  Google Scholar 

  28. Beiko, R. G., Harlow, T. J., Ragan, M. A. (2005) Highways of gene sharing in prokaryotes. Proc Natl Acad Sci U S A 102, 14332–7.

    Article  CAS  PubMed  Google Scholar 

  29. Hayashi, T., Makino, K., Ohnishi, M., Kurokawa, K., Ishii, K., Yokoyama, K., Han, C. G., Ohtsubo, E., Nakayama, K., Murata, T., Tanaka, M., Tobe, T., Iida, T., Takami, H., Honda, T., Sasakawa, C., Ogasawara, N., Yasunaga, T., Kuhara, S., Shiba, T., Hattori, M., Shinagawa, H. (2001) Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12. DNA Res 8, 11–22.

    Article  CAS  PubMed  Google Scholar 

  30. Welch, R. A., Burland, V., Plunkett, G., 3rd, Redford, P., Roesch, P., Rasko, D., Buckles, E. L., Liou, S. R., Boutin, A., Hackett, J., Stroud, D., Mayhew, G. F., Rose, D. J., Zhou, S., Schwartz, D. C., Perna, N. T., Mobley, H. L., Donnenberg, M. S., Blattner, F. R. (2002) Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli. Proc Natl Acad Sci U S A 99, 17020–4.

    Article  CAS  PubMed  Google Scholar 

  31. Eisen, J. A. (2000) Horizontal gene transfer among microbial genomes: New insights from complete genome analysis. Curr Opin Genet Dev 10, 606–11.

    Article  CAS  PubMed  Google Scholar 

  32. Koonin, E. V., Makarova, K. S., Aravind, L. (2001) Horizontal gene transfer in prokaryotes: Quantification and classification. Annu Rev Microbiol 55, 709–42.

    Article  CAS  PubMed  Google Scholar 

  33. Ragan, M. A. (2001) Detection of lateral gene transfer among microbial genomes. Curr Opin Genet Dev 11, 620–6.

    Article  CAS  PubMed  Google Scholar 

  34. Lawrence, J. G., Ochman, H. (2002) Reconciling the many faces of lateral gene transfer. Trends Microbiol 10, 1–4.

    Article  CAS  PubMed  Google Scholar 

  35. Syvanen, M. (1994) Horizontal gene transfer: Evidence and possible consequences. Annu Rev Genet 28, 237–61.

    CAS  PubMed  Google Scholar 

  36. Gaasterland, T., Ragan, M. A. (1998) Microbial genescapes: Phyletic and functional patterns of ORF distribution among prokaryotes. Microb Comp Genomics 3, 199–217.

    CAS  PubMed  Google Scholar 

  37. Tatusov, R. L., Galperin, M. Y., Natale, D. A., Koonin, E. V. (2000) The COG database: A tool for genome-scale analysis of protein functions and evolution. Nucleic Acids Res 28, 33–6.

    Article  CAS  PubMed  Google Scholar 

  38. Tatusov, R. L., Koonin, E. V., Lipman, D. J. (1997) A genomic perspective on protein families. Science 278, 631–7.

    Article  CAS  PubMed  Google Scholar 

  39. Brown, J. R., Doolittle, W. F. (1999) Gene descent, duplication, and horizontal transfer in the evolution of glutamyl- and glutaminyl-tRNA synthetases. J Mol Evol 49, 485–95.

    Article  CAS  PubMed  Google Scholar 

  40. Moreira, D., Philippe, H. (2000) Molecular phylogeny: pitfalls and progress. Int Microbiol 3, 9–16.

    CAS  PubMed  Google Scholar 

  41. Efron, B., Halloran, E., Holmes, S. (1996) Bootstrap confidence levels for phylogenetic trees. Proc Natl Acad Sci U S A 93, 13429–34.

    Article  CAS  PubMed  Google Scholar 

  42. Martin, W., Rujan, T., Richly, E., Hansen, A., Cornelsen, S., Lins, T., Leister, D., Stoebe, B., Hasegawa, M., Penny, D. (2002) Evolutionary analysis of Arabidopsis, cyanobacterial, and chloroplast genomes reveals plastid phylogeny and thousands of cyanobacterial genes in the nucleus. Proc Natl Acad Sci U S A 99, 12246–51.

    Article  CAS  PubMed  Google Scholar 

  43. Mrazek, J., Bhaya, D., Grossman, A. R., Karlin, S. (2001) Highly expressed and alien genes of the Synechocystis genome. Nucleic Acids Res 29, 1590–601.

    Article  CAS  PubMed  Google Scholar 

  44. Garcia-Vallve, S., Romeu, A., Palau, J. (2000) Horizontal gene transfer in bacterial and archaeal complete genomes. Genome Res 10, 1719–25.

    Article  CAS  PubMed  Google Scholar 

  45. Makarova, K. S., Aravind, L., Galperin, M. Y., Grishin, N. V., Tatusov, R. L., Wolf, Y. I., Koonin, E. V. (1999) Comparative genomics of the Archaea (Euryarchaeota): Evolution of conserved protein families, the stable core, and the variable shell. Genome Res 9, 608–28.

    CAS  PubMed  Google Scholar 

  46. Nesbo, C. L., Boucher, Y., Doolittle, W. F. (2001) Defining the core of nontransferable prokaryotic genes: The euryarchaeal core. J Mol Evol 53, 340–50.

    Article  CAS  PubMed  Google Scholar 

  47. Shi, T., Falkowski, P. G. (2008) Genome evolution in cyanobacteria: The stable core and the variable shell. Proc Natl Acad Sci U S A 105, 2510–5.

    Article  CAS  PubMed  Google Scholar 

  48. Tettelin, H., Masignani, V., Cieslewicz, M. J., Donati, C., Medini, D., Ward, N. L., Angiuoli, S. V., Crabtree, J., Jones, A. L., Durkin, A. S., Deboy, R. T., Davidsen, T. M., Mora, M., Scarselli, M., Margarit Y Ros, I., Peterson, J. D., Hauser, C. R., Sundaram, J. P., Nelson, W. C., Madupu, R., Brinkac, L. M., Dodson, R. J., Rosovitz, M. J., Sullivan, S. A., Daugherty, S. C., Haft, D. H., Selengut, J., Gwinn, M. L., Zhou, L., Zafar, N., Khouri, H., Radune, D., Dimitrov, G., Watkins, K., O’connor, K. J., Smith, S., Utterback, T. R., White, O., Rubens, C. E., Grandi, G., Madoff, L. C., Kasper, D. L., Telford, J. L., Wessels, M. R., Rappuoli, R., Fraser, C. M. (2005) Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae: Implications for the microbial “pan-genome”. Proc Natl Acad Sci U S A 102, 13950–5.

    Article  CAS  PubMed  Google Scholar 

  49. Makarova, K., Slesarev, A., Wolf, Y., Sorokin, A., Mirkin, B., Koonin, E., Pavlov, A., Pavlova, N., Karamychev, V., Polouchine, N., Shakhova, V., Grigoriev, I., Lou, Y., Rohksar, D., Lucas, S., Huang, K., Goodstein, D. M., Hawkins, T., Plengvidhya, V., Welker, D., Hughes, J., Goh, Y., Benson, A., Baldwin, K., Lee, J. H., Diaz-Muniz, I., Dosti, B., Smeianov, V., Wechter, W., Barabote, R., Lorca, G., Altermann, E., Barrangou, R., Ganesan, B., Xie, Y., Rawsthorne, H., Tamir, D., Parker, C., Breidt, F., Broadbent, J., Hutkins, R., O’sullivan, D., Steele, J., Unlu, G., Saier, M., Klaenhammer, T., Richardson, P., Kozyavkin, S., Weimer, B., Mills, D. (2006) Comparative genomics of the lactic acid bacteria. Proc Natl Acad Sci U S A 103, 15611–6.

    Article  PubMed  Google Scholar 

  50. Mulkidjanian, A. Y., Koonin, E. V., Makarova, K. S., Mekhedov, S. L., Sorokin, A., Wolf, Y. I., Dufresne, A., Partensky, F., Burd, H., Kaznadzey, D., Haselkorn, R., Galperin, M. Y. (2006) The cyanobacterial genome core and the origin of photosynthesis. Proc Natl Acad Sci U S A 103, 13126–31.

    Article  CAS  PubMed  Google Scholar 

  51. Kettler, G. C., Martiny, A. C., Huang, K., Zucker, J., Coleman, M. L., Rodrigue, S., Chen, F., Lapidus, A., Ferriera, S., Johnson, J., Steglich, C., Church, G. M., Richardson, P., Chisholm, S. W. (2007) Patterns and implications of gene gain and loss in the evolution of Prochlorococcus. PLoS Genet 3, e231.

    Article  PubMed  Google Scholar 

  52. Graham, D. E., Overbeek, R., Olsen, G. J., Woese, C. R. (2000) An archaeal genomic signature. Proc Natl Acad Sci U S A 97, 3304–8.

    Article  CAS  PubMed  Google Scholar 

  53. Martin, K. A., Siefert, J. L., Yerrapragada, S., Lu, Y., Mcneill, T. Z., Moreno, P. A., Weinstock, G. M., Widger, W. R., Fox, G. E. (2003) Cyanobacterial signature genes. Photosynth Res 75, 211–21.

    Article  CAS  PubMed  Google Scholar 

  54. Thompson, J. D., Higgins, D. G., Gibson, T. J. (1994) CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–80.

    Article  CAS  PubMed  Google Scholar 

  55. Nakamura, Y., Kaneko, T., Sato, S., Mimuro, M., Miyashita, H., Tsuchiya, T., Sasamoto, S., Watanabe, A., Kawashima, K., Kishida, Y., Kiyokawa, C., Kohara, M., Matsumoto, M., Matsuno, A., Nakazaki, N., Shimpo, S., Takeuchi, C., Yamada, M., Tabata, S. (2003) Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids (supplement). DNA Res 10, 181–201.

    Article  CAS  PubMed  Google Scholar 

  56. Turner, S., Pryer, K. M., Miao, V. P., Palmer, J. D. (1999) Investigating deep phylogenetic relationships among cyanobacteria and plastids by small subunit rRNA sequence analysis. J Eukaryot Microbiol 46, 327–38.

    Article  CAS  PubMed  Google Scholar 

  57. Castenholz, R. W. (1989) Order Oscillatoriales. In Bergey’s Manual of Systematic Bacteriology, Vol. 3, Williams & Wilkins, London, pp. 1771–80.

    Google Scholar 

  58. Rippka, R., Deruelles, J., Waterbury, J.B., Herdman, M., Stanier, R.Y. (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiology 111, 1–61.

    Google Scholar 

  59. Waterbury, J. B., Watson, S.W., Valois, F.W., Franks, D.G. (1986) Biological and ecological characterization of the maine unicellular cyanobacterium Synechococcus. Can Bull Fish Aquat Sci 214, 71–120.

    Google Scholar 

  60. Nelissen, B., Van De Peer, Y., Wilmotte, A., De Wachter, R. (1995) An early origin of plastids within the cyanobacterial divergence is suggested by evolutionary trees based on complete 16S rRNA sequences. Mol Biol Evol 12, 1166–73.

    CAS  PubMed  Google Scholar 

  61. Garrity, G., Boone, D. R., Castenholz, R.W. (2001) Phylum BX. Cyanobacteria, oxygenic photosynthetic bacteria. Bergey’s Manual of Systematic Bacteriology, Vol., 1, Springer, New York.

    Google Scholar 

  62. Swingley, W. D., Blankenship, R. E., Raymond, J. (2008) Integrating Markov clustering and molecular phylogenetics to reconstruct the cyanobacterial species tree from conserved protein families. Mol Biol Evol 25, 643–54.

    Article  CAS  PubMed  Google Scholar 

  63. Kumar, S., Tamura, K., Nei, M. (1994) MEGA: Molecular Evolutionary Genetics Analysis software for microcomputers. Comput Appl Biosci 10, 189–91.

    CAS  PubMed  Google Scholar 

  64. Palenik, B., Haselkorn, R. (1992) Multiple evolutionary origins of prochlorophytes, the chlorophyll b-containing prokaryotes. Nature 355, 265–7.

    Article  CAS  PubMed  Google Scholar 

  65. Urbach, E., Robertson, D. L., Chisholm, S. W. (1992) Multiple evolutionary origins of prochlorophytes within the cyanobacterial radiation. Nature 355, 267–70.

    Article  CAS  PubMed  Google Scholar 

  66. Rocap, G., Distel, D. L., Waterbury, J. B., Chisholm, S. W. (2002) Resolution of Prochlorococcus and Synechococcus ecotypes by using 16S-23S ribosomal DNA internal transcribed spacer sequences. Appl Environ Microbiol 68, 1180–91.

    Article  CAS  PubMed  Google Scholar 

  67. Fuhrman, J. (2003) Genome sequences from the sea. Nature 424, 1001–2.

    Article  CAS  PubMed  Google Scholar 

  68. Palenik, B., Brahamsha, B., Larimer, F. W., Land, M., Hauser, L., Chain, P., Lamerdin, J., Regala, W., Allen, E. E., Mccarren, J., Paulsen, I., Dufresne, A., Partensky, F., Webb, E. A., Waterbury, J. (2003) The genome of a motile marine Synechococcus. Nature 424, 1037–42.

    Article  CAS  PubMed  Google Scholar 

  69. Wommack, K. E., Colwell, R. R. (2000) Virioplankton: Viruses in aquatic ecosystems. Microbiol Mol Biol Rev 64, 69–114.

    Article  CAS  PubMed  Google Scholar 

  70. Pedulla, M. L., Ford, M. E., Houtz, J. M., Karthikeyan, T., Wadsworth, C., Lewis, J. A., Jacobs-Sera, D., Falbo, J., Gross, J., Pannunzio, N. R., Brucker, W., Kumar, V., Kandasamy, J., Keenan, L., Bardarov, S., Kriakov, J., Lawrence, J. G., Jacobs, W. R., Jr., Hendrix, R. W., Hatfull, G. F. (2003) Origins of highly mosaic mycobacteriophage genomes. Cell 113, 171–82.

    Article  CAS  PubMed  Google Scholar 

  71. Suttle, C. A. (2007) Marine viruses – major players in the global ecosystem. Nat Rev Microbiol 5, 801–12.

    Article  CAS  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Human Genome Sequencing Center Baylor College of Medicine, Houston, Texas, USA

    Shailaja Yerrapragada

  2. Department of Statistics, Rice University, Houston, TX, USA

    Janet L. Siefert

  3. Department of Biology and Biochemistry, University of Houston, Houston, TX, USA

    George E. Fox

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  1. Shailaja Yerrapragada
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  2. Janet L. Siefert
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  3. George E. Fox
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Editors and Affiliations

  1. University of Connecticut, 968 Warrenville Road, Mansfield Center, CT 06250, USA

    Maria Boekels Gogarten

  2. Dept. Molecular & Cell Biology, University of Connecticut, 91 North Eagleville Rd., Storrs, CT 06269-3125, USA

    Johann Peter Gogarten

  3. Biology Department, St. Lawrence University, 23 Romoda Drive, Canton, NY 13617, USA

    Lorraine C. Olendzenski

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Yerrapragada, S., Siefert, J.L., Fox, G.E. (2009). Horizontal Gene Transfer in Cyanobacterial Signature Genes. In: Gogarten, M.B., Gogarten, J.P., Olendzenski, L.C. (eds) Horizontal Gene Transfer. Methods in Molecular Biology, vol 532. Humana Press. https://doi.org/10.1007/978-1-60327-853-9_20

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  • DOI: https://doi.org/10.1007/978-1-60327-853-9_20

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