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Biolistic Mediated Production of Transgenic Oil Palm

  • Protocol
Advanced Protocols in Oxidative Stress I

Part of the book series: Methods In Molecular Biology ((MIMB,volume 477))

Abstract

Physical and biological parameters affecting DNA delivery into oil palm embryogenic calli using the biolistic device are optimized. Five different promoters are also evaluated to identify the most suitable promoter for use in oil palm transformation. Finally, the effectiveness of kanamycin, geneticin (G418), neomycin, hygromycin, and herbicide Basta as selection agents to inhibit growth of oil palm embryogenic calli is evaluated. Combination of optimized parameters, best promoter and selection agent is later used to transform oil palm embryogenic calli for producing transgenic oil palm plants. Bombarded embryogenic calli are exposed to 50 mg/l of Basta after 3 weeks. Basta-resistant embryogenic calli started to emerge five to six months in medium containing Basta. The Basta-resistant embryogenic calli are proliferated until they reach a specific size, and the Basta-resistant calli are later individually isolated and regenerated to produce complete plantlets. The complete regenerated plantlets are evaluated for the presence of transgenes by PCR, Southern and thin layer chromatography analyses.

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Author information

Authors and Affiliations

  1. Advanced Biotechnology and Breeding Centre, Biological Research Division, Malaysian Palm Oil Board, Kuala Lumpur, Malaysia

    Ghulam Kadir Ahmad Parveez

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  1. Ghulam Kadir Ahmad Parveez
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Editor information

Editors and Affiliations

  1. University at Buffalo, Buffalo, NY, USA

    Donald Armstrong

  2. Showa University School of Medicine, Tokyo, USA

    Donald Armstrong

  3. University of Florida, Gainesville, FL, USA

    Donald Armstrong

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Kadir Ahmad Parveez, G. (2008). Biolistic Mediated Production of Transgenic Oil Palm. In: Armstrong, D. (eds) Advanced Protocols in Oxidative Stress I. Methods In Molecular Biology, vol 477. Humana Press. https://doi.org/10.1007/978-1-60327-517-0_23

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  • DOI: https://doi.org/10.1007/978-1-60327-517-0_23

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-218-6

  • Online ISBN: 978-1-60327-517-0

  • eBook Packages: Springer Protocols

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