Summary
We present a high-resolution mass spectrometric footprinting approach enabling the identification of amino acids in the protein of interest interacting with cognate RNA. This approach is particularly attractive for studying large nucleoprotein complexes that are less amenable to crystallographic or nuclear magnetic resonance analysis. Importantly, our methodology allows examination of protein-RNA interactions under biologically relevant conditions using limited amounts of protein and nucleic acid samples.
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© 2008 Humana Press, a part of Springer Science + Business Media, LLC
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Kvaratskhelia, M., Grice, S.F.J.L. (2008). Structural Analysis of Protein-RNA Interactions With Mass Spectrometry. In: Lin, RJ. (eds) RNA-Protein Interaction Protocols. Methods in Molecular Biology, vol 488. Humana Press. https://doi.org/10.1007/978-1-60327-475-3_15
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DOI: https://doi.org/10.1007/978-1-60327-475-3_15
Publisher Name: Humana Press
Print ISBN: 978-1-58829-419-7
Online ISBN: 978-1-60327-475-3
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