Summary
A large number of proteins contain multiple RNA recognition motifs (RRMs). How multiple RRMs contribute to RNA recognition in solution is, however, poorly understood. Here, we describe a simple biochemical approach called CLAMP (crosslinking and mapping of protein domain) to identify an RRM that is crosslinked to a specific nucleotide in RNA. It involves site-specific incorporation of a chromophore, photochemical RNA-protein crosslinking, and site-specific chemical cleavage of the protein. This technique is suitable for numerous other RNA binding proteins that have multiple RNA binding domains.
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Banerjee, H., Singh, R. (2008). A Simple Crosslinking Method, CLAMP, to Map the Sites of RNA-Contacting Domains Within a Protein. In: Lin, RJ. (eds) RNA-Protein Interaction Protocols. Methods in Molecular Biology, vol 488. Humana Press. https://doi.org/10.1007/978-1-60327-475-3_12
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DOI: https://doi.org/10.1007/978-1-60327-475-3_12
Publisher Name: Humana Press
Print ISBN: 978-1-58829-419-7
Online ISBN: 978-1-60327-475-3
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