Abstract
All of the methods of enzymatic analysis to be considered depend on pyridine nucleotides. In 1935 Negelein and Hass (1) in Warburg’s laboratory described a method for the determination of glucose-6-P dehydrogenase activity based on the increase in absorption in the near UV as NADPH was produced. This is apparently the first publication of a method of this type. Greengard (2) was the first to describe fluorometric pyridine nucleotide methods for measuring metabolites. Since 1935, an enormous number of enzymes and metabolites have been measured with the aid of NAD and NADP. In fact, with the use of auxiliary enzymes, almost every substance of biological interest could be measured with a pyridine nucleotide system.
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References
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© 1993 The Humana Press Inc.
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Passonneau, J.V., Lowry, O.H. (1993). Pyridine Nucleotides. In: Enzymatic Analysis. Biological Methods. Humana Press. https://doi.org/10.1007/978-1-60327-407-4_1
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DOI: https://doi.org/10.1007/978-1-60327-407-4_1
Publisher Name: Humana Press
Print ISBN: 978-0-89603-238-5
Online ISBN: 978-1-60327-407-4
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