Abstract
Peptide arrays differ from conventional peptide synthesis in that hundreds upon thousands of peptides are synthesized and presented on a planar surface at a time. While direct synthesis of peptide arrays on a functionalized surface is feasible, reprinting of pre-made peptides offers flexibility and reproducibility and drastically reduces cost when multiple copies of the same or related peptide arrays are needed. Cellu-Spot™, a method developed by Intavis, opens a new route in peptide array synthesis and printing and overcomes certain limitations of the SPOT membrane. This technique was used to produce hundreds of phosphotyrosine-oriented peptide array libraries for determining the specificity of the Src homology 2 (SH2) domain.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Wu, C., Li, S.SC. (2009). CelluSpots™: A Reproducible Means of Making Peptide Arrays for the Determination of SH2 Domain Binding Specificity. In: Cretich, M., Chiari, M. (eds) Peptide Microarrays. Methods in Molecular Biology™, vol 570. Humana Press. https://doi.org/10.1007/978-1-60327-394-7_8
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DOI: https://doi.org/10.1007/978-1-60327-394-7_8
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Publisher Name: Humana Press
Print ISBN: 978-1-60327-393-0
Online ISBN: 978-1-60327-394-7
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