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Peptide Arrays for Enzyme Profiling

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Peptide Microarrays

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 570))

Abstract

Enzymes are key molecules in signal transduction pathways. However, only a small fraction of more than 500 predicted human kinases, 250 proteases and 250 phosphatases is characterized so far. Peptide microarray-based technologies for extremely efficient profiling of enzyme substrate specificity emerged in the last years. Additionally, patterns of enzymatic activities could be used to fingerprint the status of cells or organisms. This technology reduces set-up time for HTS assays and allows the identification of downstream targets. Moreover, peptide microarrays enable optimization of enzyme substrates. A comprehensive overview regarding enzyme profiling using peptide microarrays is presented with special focus on assay principles.

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Authors and Affiliations

  1. Max Planck Research Unit for Enzymology of Protein Folding, Halle, Germany

    Alexandra Thiele

  2. JPT Peptide Technologies GmbH, Berlin, Germany

    Johannes Zerweck & Mike Schutkowski

Authors
  1. Alexandra Thiele
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  2. Johannes Zerweck
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  3. Mike Schutkowski
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Editors and Affiliations

  1. Inst. Chimica del Riconoscimento, CNR, Via Mario Bianco 9, Milano, 20131, Italy

    Marina Cretich

  2. Inst. Chimica del Riconoscimento, CNR, Via Mario Bianco 9, Milano, 20131, Italy

    Marcella Chiari

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Thiele, A., Zerweck, J., Schutkowski, M. (2009). Peptide Arrays for Enzyme Profiling. In: Cretich, M., Chiari, M. (eds) Peptide Microarrays. Methods in Molecular Biology™, vol 570. Humana Press. https://doi.org/10.1007/978-1-60327-394-7_2

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