Abstract
It is extremely useful to define a rapid and accurate method for identifying homozygous and heterozygous transgenic animals prior to setting up breeding programs for transgenic colonies and in experiments in which gene dosage effects could have a functional impact. Southern-blotting is a means of identifying zygosity, but such a method is time consuming and produces a high level of ambiguous results. Some years ago, we described the rapid, precise, non-ambiguous, and high-throughput identification of zygosity in transgenic animals by real-time PCR. This technique allows us to make a clear-cut identification of transgenic rats, transgenic mice, and double-transgenic pigs. Since 2002, however, several authors have made improvements to this method. The following paper describes the ease with which zygosity is determined using real-time PCR.
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References
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© 2010 Humana Press, a part of Springer Science+Business Media, LLC
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Tesson, L., Rémy, S., Ménoret, S., Usal, C., Anegon, I. (2010). Analysis by Quantitative PCR of Zygosity in Genetically Modified Organisms. In: Anegon, I. (eds) Rat Genomics. Methods in Molecular Biology, vol 597. Humana Press. https://doi.org/10.1007/978-1-60327-389-3_19
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DOI: https://doi.org/10.1007/978-1-60327-389-3_19
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