Abstract
Somatic cell nuclear transfer (SCNT) is a powerful tool for the investigation of the mechanisms of nuclear remodeling. In addition, SCNT may offer the possibility of introducing targeted mutations by homologous recombination in species for which ES cell technology is not available. The rat specific features of the oocyte have long impeded the development of SCNT. We detail here the procedures developed and optimized during the last several years for the optimization of rat cloning.
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Acknowledgments
This work was supported by grants from ANR “ANR-05-PRIB-026” and the EuraTool project (European Rat Tools for Functional Genomics).
The author would like to thank staff from the embryology and molecular biology department for their technical help in writing the document.
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Cozzi, J., Wang, E., Jacquet, C., Fraichard, A., Cherifi, Y., Zhou, Q. (2010). Procedures for Somatic Cell Nuclear Transfer in the Rat. In: Anegon, I. (eds) Rat Genomics. Methods in Molecular Biology, vol 597. Humana Press. https://doi.org/10.1007/978-1-60327-389-3_10
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DOI: https://doi.org/10.1007/978-1-60327-389-3_10
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