Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
The International SNP Map Working Group (2001) A map of human genome sequence variation containing 1.42 million single nucleotide polymorphisms. Nature 49:928–933
Cotton RGH (1997) Mutation Detection. Oxford University Press, Oxford
Xiao W, Stern D, Jain M, Huber CG, Oefner PJ (2001) Multiplex capillary denaturing high-performance liquid chromatography with laser-induced fluorescence detection. Biotechniques 30:1332–1338
Xiao WH, Oefner PJ (2001) Denaturing high-performance liquid chromatography: a review. Hum Mutat 17:439–474
Gjerde DT, Hanna CP, Hornby D (2002) DNA chromatography. Wiley-VCH, Weinheim
Hung CC, Su YN, Lin CY, Yang CC, Lee WT, Chien SC, Lin WL, Lee CN (2005) Denaturing HPLC coupled with multiplex PCR for rapid detection of large deletions in Duchenne muscular dystrophy carriers. Clin Chem 51:1252–1256
Dehainault C, Lauge A, Caux-Moncoutier V, Pages-Berhouet S, Doz F, Desjardins L, Couturier J, Gauthier-Villars M, Stoppa-Lyonnet D, Houdayer C (2004) Multiplex PCR/liquid chromatography assay for detection of gene rearrangements: application to RB1 gene. Nucleic Acids Res 32:e139
Han W, Yip SP, Wang J, and Yap MKH (2004) Using denaturing HPLC for SNP discovery and genotyping, and establishing the linkage disequilibrium pattern for the all-trans-retinol dehydrogenase (RDH8) gene. J Hum Genet 49:16–23
Ravnik-Glavac M, Atkinson A, Glavac D, Dean M (2002) DHPLC screening of cystic fibrosis gene mutations. Hum Mutat 19:374–383
Yip SP, Pun SF, Leung KH, Lee SY (2003) Rapid simultaneous genotyping of five common Southeast Asian ß-thalassemia mutations by multiplex minisequencing and denaturing HPLC. Clin Chem 49:1656–1659
Chu MY (2003) Mutational analysis of the RLBP1 and CHM genes in Chinese patients with retinal degeneration. MSc Dissertation. The Hong Kong Polytechnic University, Hong Kong SAR, China
Colosimo A, Guida V, De Luca A, Cappabianca MP, Bianco I, Palka G, and Dallapiccola B. (2002) Reliability of DHPLC in mutational screening of betaglobin (HBB) alleles. Hum Mutat 19:287–295
Lilleberg SL, Durocher J, Sanders C, Walters K, Culver K (2004) High sensitivity scanning of colorectal tumors and matched plasma DNA for mutations in APC, TP53, K-RAS, and BRAF genes with a novel DHPLC fluorescence detection platform. Ann NY Acad Sci 1022:250–256
zur Stadt U, Rischewski J, Schneppenheim R, Kabisch H (2001) Denaturing HPLC for identification of clonal T-cell receptor gamma rearrangements in newly diagnosed acute lymphoblastic leukemia. Clin Chem 47:2003–2011
Hoogendoorn B, Owen MJ, Oefner PJ, Williams N, Austin J, O'Donovan MC (1999) Genotyping single nucleotide polymorphisms by primer extension and high performance liquid chromatography. Hum Genet 104:89–93
Liang Q, Davis PA, Thompson BH, Simpson JT (2001) High-performance liquid chromatography multiplex detection of two single nucleotide mutations associated with hereditary hemochromatosis. J Chromatogr B Biomed Sci Appl 754: 265–270
Devaney JM, Pettit EL, Kaler SG, Vallone PM, Butler JM, Marino MA (2001) Genotyping of two mutations in the HFE gene using single-base extension and high-performance liquid chromatography. Anal Chem 73:620–624
Wu G, Hua L, Zhu J, Mo QH, Xu XM (2003) Rapid, accurate genotyping of beta-thalassaemia mutations using a novel multiplex primer extension/denaturing high-performance liquid chromatography assay. Br J Haematol 122:311–316
Hoogendoorn B, Norton N, Kirov G, Williams N, Hamshere ML, Spurlock G, Austin J, Stephens MK, Buckland PR, Owen MJ, O'Donovan MC (2000) Cheap, accurate and rapid allele frequency estimation of single nucleotide polymorphisms by primer extension and DHPLC in DNA pools. Hum Genet 107:488–493
Lewis CM (2002) Genetic association studies: design, analysis and interpretation. Brief Bioinform 3:146–153
Norton N, Williams NM, O'Donovan MC, Owen MJ (2004) DNA pooling as a tool for large-scale association studies in complex traits. Ann Med 36:146–152
Hannachi-M'Zali F, Ambler JE, Taylor CF, Hawkey PM (2002) Examination of single and multiple mutations involved in resistance to quinolones in Staphylococcus aureus by a combination of PCR and denaturing high-performance liquid chroma-tography (DHPLC). J Antimicrob Chemother 50:649–655
Eaves DJ, Liebana E, Woodward MJ, Piddock LJ (2002) Detection of gyrA mutations in quinolone-resistant Salmonella enterica by denaturing high-performance liquid chromatography. J Clin Microbiol 40:4121–4125
Randall LP, Coldham NG, Woodward MJ (2005) Detection of mutations in Salmonella enterica gyrA, gyrB, parC and parE genes by denaturing high performance liquid chromatography (DHPLC) using standard HPLC instrumentation. J Antimicrob Chemother 56:619–623
Hurtle W, Lindler L, Fan W, Shoemaker D, Henchal E, Norwood D (2003) Detection and identification of ciprofloxacin-resistant Yersinia pestis by denaturing high-performance liquid chromatography. J Clin Microbiol 41:3273–3283
Perez-Perez FJ Hanson ND (2002) Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR. J Clin Microbiol 40: 2153–2162
Shi R, Otomo K, Yamada H, Tatsumi T, Sugawara I (2006) Temperature-mediated heteroduplex analysis for the detection of drug-resistant gene mutations in clinical isolates of Mycobacterium tuberculosis by denaturing HPLC, SURVEYOR nucle-ase. Microbes Infect 8:128–135
Hurtle W, Shoemaker D, Henchal E, Norwood D (2002) Denaturing HPLC for identifying bacteria. Biotechniques 33:386–391
Evans JT, Hawkey PM, Smith EG, Boese KA, Warren RE, Hong G (2004) Automated high-throughput mycobacterial interspersed repetitive unit typing of Mycobacterium tuberculosis strains by a combination of PCR and nondenaturing high-performance liquid chromatography. J Clin Microbiol 42:4175–4180
Goldenberg O, Herrmann S, Adam T, Marjoram G, Hong G, Gobel UB, Graf B (2005) Use of denaturing high-performance liquid chromatography for rapid detection and identification of seven Candida species. J Clin Microbiol 43:5912–5915
Domann E, Hong G, Imirzalioglu C, Turschner S, Kuhle J, Watzel C, Hain T, Hossain H, Chakraborty T (2003) Culture-independent identification of pathogenic bacteria and polymicrobial infections in the genitourinary tract of renal transplant recipients. J Clin Microbiol 41:5500–5510
Goldenberg O, Herrmann S, Marjoram G, Noyer-Weidner M, Hong G, Bereswill S, Gobel UB (2007) Molecular monitoring of the intestinal flora by denaturing high performance liquid chromatography. J Microbiol Methods (in press)
Barlaan EA, Sugimori M, Furukawa S, Takeuchi K (2005) Profiling and monitoring of microbial populations by denaturing high-performance liquid chromatogra-phy. J Microbiol Methods 61:399–412
Becker-Andre M, Hahlbrock K (1989) Absolute mRNA quantification using the polymerase chain reaction (PCR). A novel approach by a PCR aided transcript titration assay (PATTY). Nucleic Acids Res 17: 9437–9446
Freeman WM, Walker SJ, Vrana KE (1999) Quantitative RT-PCR: pitfalls and potential. Biotechniques 26:112–125
Leerkes MR, Caballero OL, Mackay A, Torloni H, O'Hare MJ, Simpson AJ, de Souza SJ (2002) In silico comparison of the transcriptome derived from purified normal breast cells and breast tumor cell lines reveals candidate upregulated genes in breast tumor cells. Genomics 79:257–265
Hayward AL, Hinojos CA, Nurowska B, Hewetson A, Sabatini S, Oefner PJ, Doris PA (1999) Altered sodium pump alpha and gamma subunit gene expression in nephron segments from hypertensive rats. J Hypertens 17:1081–1087
Hecker KH, Green SM, Kobayashi K (2000) Analysis and purification of nucleic acids by ion-pair reversed-phase high-performance liquid chromatography. J Biochem Biophys Methods 46:83–93
Dickman M, Hornby DP (2000) Isolation of single-stranded DNA using denaturing DNA chromatography. Anal Biochem 284:164–167
Azarani A, Hecker KH (2001) RNA analysis by ion-pair reversed-phase high performance liquid chromatography. Nucleic Acids Res 29:e7
Robertson KD, Wolffe AP (2000) DNA methylation in health and disease. Nat Rev Genet 1:11–19
Frommer M, McDonald LE, Millar DS, Collis CM, Watt F, Grigg GW, Molloy PL, Paul CL (1992) A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands. Proc Natl Acad Sci USA 89: 1827–1831
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Singer-Sam J, Grant M, LeBon JM, Okuyama K, Chapman V, Monk M, Riggs AD (1990) Use of a HpaII-polymerase chain reaction assay to study DNA methylation in the Pgk-1 CpG island of mouse embryos at the time of X-chromosome inactiva-tion. Mol Cell Biol 10:4987–4989
Baumer A (2002) Analysis of the methylation status of imprinted genes based on methylation-specific polymerase chain reaction combined with denaturing high-performance liquid chromatography. Methods 27:139–143
El-Maarri O, Herbiniaux U, Walter J, Oldenburg J (2002) A rapid, quantitative, non-radioactive bisulfite-SNuPE-IP RP HPLC assay for methylation analysis at specific CpG sites. Nucleic Acids Res 30:e25
Couvert P, Poirier K, Carrie A, Chalas C, Jouannet P, Beldjord C, Bienvenu T, Chelly J, Kerjean A (2003) DHPLC-based method for DNA methylation analysis of differential methylated regions from imprinted genes. Biotechniques 34: 356–362
Holland MM, Parsons TJ (1999) Mitochondrial DNA Sequence Analysis: validation and use for forensic casework. Forensic Sci Rev 11:21–50
LaBerge GS, Shelton RJ, Danielson PB (2003) Forensic utility of mitochondrial DNA analysis based on denaturing high-performance liquid chromatography. Croat Med J 44:281–288
Acknowledgments
Work with DHPLC was funded by the following research grants: PolyU Big Equipment Grant (G.53.27.9027), PolyU Central Research Grants (G-YD47, G-YD74 and G-U069), Dean's Reserve (1.53.09.87AV) and RGC Competitive Earmarked Research Grant (B-Q04A).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2008 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Leung, K.H., Yip, S.P. (2008). Denaturing High-Performance Liquid Chromatography (DHPLC) for Nucleic Acid Analysis. In: Walker, J.M., Rapley, R. (eds) Molecular Biomethods Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-375-6_7
Download citation
DOI: https://doi.org/10.1007/978-1-60327-375-6_7
Publisher Name: Humana Press
Print ISBN: 978-1-60327-370-1
Online ISBN: 978-1-60327-375-6
eBook Packages: Springer Protocols