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Molecular Typing Methods for Clostridium difficile: Pulsed-Field Gel Electrophoresis and PCR Ribotyping

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Clostridium difficile

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 646))

Abstract

Molecular typing methods for Clostridium difficile are based on gel electrophoresis of restriction fragments (endonuclease restriction analysis, REA; pulsed field gel electrophoresis PFGE; toxinotyping), PCR amplification (PCR ribotyping, arbitrarily primed PCR, multilocus variable-number tandem-repeat analysis MLVA), and sequence analysis (multilocus sequence typing MLST; slpA typing, tandem repeat sequence typing). We will describe two standard methods (PCR ribotyping predominantly used throughout Europe and PFGE which is predominantly used in North America) and will discuss the difficulties of inter-laboratory comparability and unification of typing nomenclature.

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Authors and Affiliations

  1. Institute of Public Health Maribor, Centre for Microbiology, Maribor, Slovenia

    Sandra Janezic & Maja Rupnik

  2. Faculty of Medicine, University of Maribor, Maribor, Slovenia

    Sandra Janezic & Maja Rupnik

Authors
  1. Sandra Janezic
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  2. Maja Rupnik
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Correspondence to Maja Rupnik .

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Editors and Affiliations

  1. Eastman Dental Institute Division of Microbial Diseases, University College, London, 256 Gray’s Inn Road, London, WC1X 8LD, UK

    Peter Mullany  & Adam P. Roberts  & 

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Janezic, S., Rupnik, M. (2010). Molecular Typing Methods for Clostridium difficile: Pulsed-Field Gel Electrophoresis and PCR Ribotyping. In: Mullany, P., Roberts, A.P. (eds) Clostridium difficile. Methods in Molecular Biology™, vol 646. Humana Press. https://doi.org/10.1007/978-1-60327-365-7_4

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  • DOI: https://doi.org/10.1007/978-1-60327-365-7_4

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-364-0

  • Online ISBN: 978-1-60327-365-7

  • eBook Packages: Springer Protocols

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