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Regulation of Inter- and Intramolecular Interaction of RNA, DNA, and Proteins by MLE

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Helicases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 587))

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Abstract

Drosophila maleless (MLE) is a member of helicase superfamily 2 and functions as a dosage compensation factor essential for the development of male flies. This function provides a good opportunity to investigate diverse biochemical activities associated with MLE in the context of a defined in vivo pathway, i.e., the transcriptional activation of X-linked genes. We have shown for the first time that MLE catalyzes the unwinding of both DNA and RNA and that MLE helicase activity is essential for its in vivo function. Also, we have provided evidence that MLE stimulates the transcriptional activity of roX2 on the X chromosome. We have also found that MLE interacts with dsDNA, topoisomerase II, and nucleosome. This observation supports a current model of dosage compensation: transcriptional activation of X-linked genes is causally associated with conformational change in the male X chromosome, subsequent to the targeted association of the dosage compensation complex (DCC).

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Author information

Authors and Affiliations

  1. HHMI, Waksman Institute, Rutgers University, Piscataway, New Jersey, US

    Hyangyee Oh

  2. Department Of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark, New Jersey, US

    Andrew M. Parrott & Chee-Gun Lee

  3. Department Of cell Biology and Molecular Medicine, UMDNJ-New Jersey Medical School, Newark, New Jersey, US

    Yongkyu Park

Authors
  1. Hyangyee Oh
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  2. Andrew M. Parrott
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  3. Yongkyu Park
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  4. Chee-Gun Lee
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Corresponding author

Correspondence to Chee-Gun Lee .

Editor information

Editors and Affiliations

  1. Dept. Paediatric Laboratory Medicine, Hospital for Sick Children, University Ave. 555, Toronto, M5G 1X8, Ontario, Canada

    Mohamed M. Abdelhaleem

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Oh, H., Parrott, A.M., Park, Y., Lee, CG. (2009). Regulation of Inter- and Intramolecular Interaction of RNA, DNA, and Proteins by MLE. In: Abdelhaleem, M. (eds) Helicases. Methods in Molecular Biology, vol 587. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-355-8_22

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  • DOI: https://doi.org/10.1007/978-1-60327-355-8_22

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-354-1

  • Online ISBN: 978-1-60327-355-8

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