Abstract
Because of the outstanding ability of two-dimensional electrophoresis to separate complex mixtures of intact proteins, it would be advantageous to apply it to all types of proteins, including hydrophobic and membrane proteins. Unfortunately, poor solubility hampers the analysis of these molecules. As these problems arise mainly in the extraction and isoelectric focusing steps, the solution is to improve protein solubility under the conditions prevailing during isoelectric focusing. This chapter describes the use of chaotropes and novel detergents to enhance protein solubility during sample extraction and isoelectric focussing, and discusses the contribution of these compounds to improving proteomic analysis of membrane proteins.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Rabilloud, T. (2009). Detergents and Chaotropes for Protein Solubilization Before Two-Dimensional Electrophoresis. In: Peirce, M.J., Wait, R. (eds) Membrane Proteomics. Methods in Molecular Biology™, vol 528. Humana Press. https://doi.org/10.1007/978-1-60327-310-7_18
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DOI: https://doi.org/10.1007/978-1-60327-310-7_18
Publisher Name: Humana Press
Print ISBN: 978-1-60327-309-1
Online ISBN: 978-1-60327-310-7
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