Summary
We provide procedures for the panning of fully humanized Fab antibodies using guided selection. Human heavy and light chain genes are amplified. A parental light chain is cloned into a phage display vector and combined with the heavy chain library. After several rounds of panning, positive clones are identified and the heavy chain sequences that are recovered are combined with light chains for further selection by phage display. Human Fab antibodies are obtained that bind the same epitope as the parental antibody.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Guo-qiang, B., Xian-Li, H. (2009). Guided Selection Methods Through Chain Shuffling. In: Aitken, R. (eds) Antibody Phage Display. Methods in Molecular Biology, vol 562. Humana Press. https://doi.org/10.1007/978-1-60327-302-2_10
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DOI: https://doi.org/10.1007/978-1-60327-302-2_10
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Online ISBN: 978-1-60327-302-2
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