Summary
Echinacace purpurea (purple cone flower) is an important medicinal plant, and widely used for phytochemical purposes. The roots are traditionally used in herbal medicines and dietary supplements as an immunostimulant in treating inflammatory and viral diseases. Extensive research work has been carried out on both the induction of adventitious roots from E. purpurea as well as established small-scale (shake flask) to large-scale (bioreactor) cultures for the production of adventitious root biomass and caffeic acid derivatives. This chapter describes the methodologies of induction of adventitious roots from explants of E. purpurea, propagation of adventitious roots in suspension cultures, estimation of total phenolics, flavonoids, and antioxidant activities. The detailed methodology for high-performance liquid chromatographic analysis of caffeic acid derivatives present in the adventitious roots is also discussed.
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References
Barrett, B. (2003). Medicinal properties of Echinacea: a critical review. Phytomedicine 10, 66–68
Bauer, R. (1999). Chemistry, analysis and immunological investigations of Echinacea Phytochemicals, in Immunomodulatory Agents from Plants (Wagner, H., ed.), BirkhauserVerlag, Basel, pp. 41–88
Rao, S. R., and Ravishanakar, G. A. (2002). Plant cell cultures: chemical factories of secondary metabolites. Biotechnol. Adv. 20, 101–153
Sajc, L., Grubiscic, D., and Vunjak-Novakovic, G. (2000) Bioreactors for plant engineering: an outlook for further research. Biochem. Eng. J. 4, 89–99
Zhong, J. J. (2001). Biochemical engineering of the production of plant-specific secondary metabolites by cell suspension cultures, in Advances in Biochemical Engineering/Biotechnology, Vol. 72 (Scheper, T., ed.), Springer-Verlag, Heidelberg, pp. 1–26
Paek, K. Y., Hahn, E. J., and Son, S. H. (2001). Application of bioreactors for large scale micropropagation systems of plants. In Vitro Cell. Dev. Biol. Plant. 37, 149–157
Wu, C. H., Murthy, H. N., Hanh, E. J., and Paek, K. Y. (2007). Large scale cultivation of adven-titious roots of Echinacea purpurea in airlift bioreactors for the production of chichoric acid, chlrogeneic acid and caftaric acid. Biotechnol. Lett. 29, 1179–1182
Wu, C. H., Murthy, H. N., Hahn, E. J., and Paek, K. Y. (2007). Improved production of caffeic acid derivatives in suspension cultures of Echinacea purpurea by medium replenishment strategy. Arch. Pharm. Res. 30, 945–949
Wu, C. H., Murthy, H. N., Hahn, E. J., and Paek, K. Y. (2007). Enhanced production of caftaric acid, chlorogenic acid and cichoric acid in suspension cultures of Echinacea purpurea by the manipulation of incubation temperature and photoperiod. Biochem. Eng. J. 36, 301–303
Murashige, T., and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15, 473–497
Stuart, D. L., and Wills, R. B. H. (2003). Effect of drying temperature on alkylamides and cichoric acid concentrations of Echinacea pupurea. J. Agric. Food Chem. 51, 1608–1610
Mabry, T. J., Markham, K. R., and Thomas, M. B. (ed.) (1970). The Systematic Identification of Flavonoids. Springer-Verlag, New York
Brand-Williams, W., Cuvelier, M. E., and Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. Lebensmittel – Wissenschaft Technologie, 28, 25–30
Acknowledgments
This work was supported partially by Korean Science and Engineering Foundation (KOSEF). Dr. H.N. Murthy is thankful to Korean Federation of Science and Technology Societies (KOFST) for the award of Brain Pool Fellowship.
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Paek, KY., Murthy, H., Hahn, EJ. (2009). Establishment of Adventitious Root Cultures of Echinacea purpurea for the Production of Caffeic Acid Derivatives. In: Jain, S.M., Saxena, P.K. (eds) Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants. Methods in Molecular Biology, vol 547. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-287-2_1
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DOI: https://doi.org/10.1007/978-1-60327-287-2_1
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