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Stages in ELISA

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The ELISA Guidebook

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 516))

This chapter gives general information on essential practical features of ELISAs. These can be summarized as follows:

  1. 1.

    Adsorption of antigen or antibody to the plastic solid phase

  2. 2.

    Addition of the test sample and subsequent reagents

  3. 3.

    Incubation of reactants

  4. 4.

    Separation of bound and free reactants by washing

  5. 5.

    Addition of enzyme-labeled reagent

  6. 6.

    Addition of enzyme detection system (color development)

  7. 7.

    Visual or spectrophotometric reading of the assay

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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(2009). Stages in ELISA. In: The ELISA Guidebook. Methods in Molecular Biology™, vol 516. Humana Press. https://doi.org/10.1007/978-1-60327-254-4_3

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  • DOI: https://doi.org/10.1007/978-1-60327-254-4_3

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-253-7

  • Online ISBN: 978-1-60327-254-4

  • eBook Packages: Springer Protocols

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