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Elucidating the Role of Metals in Alzheimer’s Disease Through the Use of Surface-Enhanced Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry

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Protein Folding, Misfolding, and Disease

Part of the book series: Methods in Molecular Biology ((MIMB,volume 752))

Abstract

Alzheimer’s disease (AD) is a highly heterogeneous and progressive dementia which is characterised by a progressive decline in cognitive functioning, selective neuronal atrophy, and loss of cortical volume in areas involved in learning and memory. However, recent research has indicated that the AD-affected brain is also besieged by increases in oxidative stress as well as perturbations to the homeostasis of biometals, such as copper and iron. These metals are known to interact with the neuropathological hallmark of AD, the β-amyloid peptide (Aβ), in a manner which increases Aβ’s neurotoxic effects. This knowledge has led to the development of therapeutic measures which act to restore biometal homeostasis within the AD brain. This chapter outlines how Surface-Enhanced Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry can be used to monitor Aβ levels within biological systems as well as describing the use of immobilised metal affinity capture in the observation of synthetic Aβ peptides.

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Author information

Authors and Affiliations

  1. Department of Pathology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia

    Andrew D. Watt

Authors
  1. Andrew D. Watt
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  2. Keyla A. Perez
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  3. Lin Wai Hung
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Corresponding author

Correspondence to Andrew D. Watt .

Editor information

Editors and Affiliations

  1. Bio21 Molecular Science and, Biotechnology Institute, University of Melbourne, Flemington Road 30, Parkville, 3010, Victoria, Australia

    Andrew F. Hill

  2. Bio21 Molecular Science and, Biotechnology Institute, University of Melbourne, Flemington Road 30, Parkville, 3010, Victoria, Australia

    Kevin J. Barnham

  3. , Department of Biochemistry &, Monash University, Wellington Road, Clayton, 3800, Victoria, Australia

    Stephen P. Bottomley

  4. Bio21 Molecular Science and, Biotechnology Institute, University of Melbourne, Flemington Road 30, Parkville, 3010, Victoria, Australia

    Roberto Cappai

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Watt, A.D., Perez, K.A., Hung, L.W. (2011). Elucidating the Role of Metals in Alzheimer’s Disease Through the Use of Surface-Enhanced Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry. In: Hill, A., Barnham, K., Bottomley, S., Cappai, R. (eds) Protein Folding, Misfolding, and Disease. Methods in Molecular Biology, vol 752. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-223-0_15

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  • DOI: https://doi.org/10.1007/978-1-60327-223-0_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-221-6

  • Online ISBN: 978-1-60327-223-0

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