Abstract
Candida albicans is a major fungal systemic pathogen in humans. Genetic manipulation of C. albicans is unwieldy. We report here a strategy that is useful and successful for large-scale genetic manipulation of C. albicans genes of interest: use of the UAU1 cassette on a Tn7 transposon. Streamlined yet admittedly flawed disruption techniques, such as the one described here, may prove vital to uncovering the genetic basis of fungal virulence.
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References
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Nobile, C.J., Mitchell, A.P. (2009). Large-Scale Gene Disruption Using the UAU1 Cassette. In: Cihlar, R.L., Calderone, R.A. (eds) Candida albicans. Methods in Molecular Biology, vol 499. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-151-6_17
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DOI: https://doi.org/10.1007/978-1-60327-151-6_17
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-58829-760-0
Online ISBN: 978-1-60327-151-6
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