Abstract
This chapter describes methods for studying downstream events of the PI3 K/Akt signaling cascade, focusing on the FoxO transcription factors. These approaches also represent alternative means for gauging the phosphoinositide-3 kinase/Akt activity. We describe protocols for the fractionation of cytoplasmic and nuclear protein extracts and for studying transcription factor DNA-binding activity in vitro and in vivo.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Essafi, A. et al. (2009). Studying the Subcellular Localization and DNA-Binding Activity of FoxO Transcription Factors, Downstream Effectors of PI3K/Akt. In: Larijani, B., Woscholski, R., Rosser, C. (eds) Lipid Signaling Protocols. Methods in Molecular Biology, vol 462. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-115-8_13
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DOI: https://doi.org/10.1007/978-1-60327-115-8_13
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Publisher Name: Humana Press, Totowa, NJ
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