Skip to main content
SpringerLink
Log in

Gene Gun Delivery Systems for Cancer Vaccine Approaches

  • Protocol
  • First Online:
Gene Therapy of Cancer

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 542))

Summary

Gene-based immunization with transgenic DNA vectors expressing tumor-associated antigens (TAA), cytokines, or chemokines, alone or in combination, provides an attractive approach to increase the cytotoxic T cell immunity against various cancer diseases. With this consideration, particle-mediated or gene gun technology has been developed as a nonviral method for gene transfer into various mammalian tissues. It has been shown to induce both humoral and cell-mediated immune responses in both small and large experimental animals. A broad range of somatic cell types, including primary cultures and established cell lines, has been successfully transfected ex vivo or in vitro by gene gun technology, either as suspension or adherent cultures. Here, we show that protocols and techniques for use in gene gun-mediated transgene delivery system for skin vaccination against melanoma using tumor-associated antigen (TAA) human gp100 and reporter gene assays as experimental systems.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Condon, C., Watkins, S.C., Celluzzi, C.M., Thompson, K., and Falo, L.D. Jr. (1996). DNA-based immunization by in vivo transfection of dendritic cells. Nat. Med. 2, 1122–1128.

    Article  PubMed  CAS  Google Scholar 

  2. Yang, N.S., Burkholder, J., Roberts, B., Martinell, B., and McCabe, D. (1990). In vivo and in vitro gene transfer to mammalian somatic cells by particle bombardment. Proc. Natl. Acad. Sci. USA 87, 9568–9572.

    Article  PubMed  CAS  Google Scholar 

  3. Cheng, L., Ziegelhoffer, P., and Yang, N.S. (1993). In vivo promoter activity and transgenic expression in mammalian somatic tissues evaluated by using particle bombardment. Proc. Natl. Acad. Sci. USA 90, 4454–4459.

    Article  Google Scholar 

  4. Pertmer, T.M., Eisenbraun, M.D., McCabe, D., Prayaga, S.K., Fuller, D.H., and Haynes, J.R. (1995). Gene gun-based nucleic acid immunization: elicitation of humoral and cytotoxic T lymphocyte responses following epidermal delivery of nanogram quantities of DNA. Vaccine 13, 1427–1430.

    Article  PubMed  CAS  Google Scholar 

  5. Feltquate, D.M., Heaney, S., Webster, R.G., and Robinson, H.L. (1997). Different T helper cell types and antibody isotypes generated by saline and gene gun DNA immunization. J. Immunol. 158, 2278–2284.

    PubMed  CAS  Google Scholar 

  6. Rakhmilevich, A. L., Turner, J., Ford, M. J., McCabe, D., Sun, W. H., Sondel, P. M., et al. (1996). Gene gun-mediated skin transfection with interleukin 12 gene results in regression of established primary and metastatic murine tumors. Proc. Natl. Acad Sci USA 93, 6291–6296.

    Article  PubMed  CAS  Google Scholar 

  7. Fuller, D.H., Corb, M.M., Barnett, S., steamer, K., and Haynes, J.R. (1997). Enhancement of immunodeficiency virus-specific immune responses in DNA-immunized rhesus macaques. Vaccine 15, 924–926.

    Article  PubMed  CAS  Google Scholar 

  8. Yamano, T., Kaneda, Y., Huang, S., Hiramatsu, S.H., and Hoon, D.S.B. (2006). Enhancement of immunity by a DNA melanoma vaccine against TRP2 with CCL21 as an adjuvant. Mol. Ther. 13, 194–202.

    Article  PubMed  CAS  Google Scholar 

  9. Yang, S.C., Batra, R.K., Hillinger, S., Reckamp, K.L., Strieter, R.M., Dubinett, S.M.et al. (2006). Intrapulmonary administration of CCL21 gene-modified dendritic cells reduced tumor burden in spontaneous murine bronchoalveolar cell carcinoma. Cancer Res. 66, 3205–3213.

    Article  PubMed  CAS  Google Scholar 

  10. Lian, H., Jin, N., Li, X., Mi, Z., Zhang, J., Sun, L. et al., (2007). Induction of an effective anti-tumor immune response and tumor regression by combined administration of IL-18 and apoptin. Cancer Immunol. Immunother. 56, 181–192.

    Article  PubMed  CAS  Google Scholar 

  11. Mahvi, D.M., Sondel, P.M., Yang, N.S., Albertini, M.R., Schiller, J.H., Hank, J., et al. (1997). Phase I/IB study of immunization with autologous tumor cells transfected with the GM-CSF gene by particle-mediated transfer in patients with melanoma or sarcoma. Hum. Gene Ther. 8, 875–891.

    Article  PubMed  CAS  Google Scholar 

  12. Cassaday, R.D., Sondel, P.M., King, D.M., Macklin, M.D., Gan, J., Warner, T.F., et al. (2007). A phase I study of immunization using particle-mediated epidermal delivery of genes for gp100 and GM-CSF into uninvolved skin of melanoma patients. Clin. Cancer Res. 13, 540–549.

    Article  PubMed  CAS  Google Scholar 

  13. Aravindaram, K., Yu, H.H., Lan, C.W., Wang, P.H., Chen, Y.H., Chen, H.M. et al. (2009). Transgenic expression of human gp 100 and RANTES at specific time points for suppression of melanoma. Gene Ther. (under revision).

    Google Scholar 

  14. Qiu, P., Ziegelhoffer, P., Sun, J., and Yang, N.S. (1996). Gene gun delivery of mRNA in situ result in efficient transgene expression and immunization. Gene Ther. 3, 262–268.

    PubMed  CAS  Google Scholar 

  15. Jiao, S., Cheng, L., Wolff, J., and Yang, N.S. (1993). Particle bombardment mediated gene transfer and expression in rat brain tissues. Bio/Technology 11, 497–502.

    Article  PubMed  CAS  Google Scholar 

  16. Burkholder, J.K., Decker, J., and Yang, N.S. (1993). Transgene expression in lymphocyte and macrophage primary cultures after particle bombardment. J. Immunol. Methods 165, 149–156.

    Article  PubMed  CAS  Google Scholar 

  17. Christou, P. (1994). Application to plants, in Particle Bombardment Technology for Gene Transfer (Yang, N.S. and Christou, P., eds.), Oxford University Press, New York, pp. 71–99.

    Google Scholar 

  18. Yang, N.S. and Ziegelhoffer, P. (1994). The particle bombardment system for mammalian gene transfer, in Particle Bombardment Technology for Gene Transfer (Yang, N.S. and Christou, P., eds.), Oxford University Press, New York, pp. 117–141.

    Google Scholar 

  19. Rakhmilevich, A.L., Imboden, M., Hao, Z., Macklin, M.D., Roberts, T., Wright, K.M., et al. (2001). Effective particle-mediated vaccination against mouse melanoma by coadministration of plasmid DNA encoding gp100 and granulocyte-macrophage colony-stimulating factor. Clin. Cancer Res. 7, 952–961.

    PubMed  CAS  Google Scholar 

  20. Yang, N.S., Wang, J.H., Lin, K.F., Wang, C.Y., Kim, S.A., Yang, Y.L., et al. (2005). Comparative studies of the capsid precursor polypeptide P1 and the capsid protein VP1 cDNA vectors for DNA vaccination against foot-and-mouth disease virus. J. Gene Med. 7, 708–717.

    Article  PubMed  CAS  Google Scholar 

  21. Guo, Z., Yang, N.S., Jiao, S., Sun, J., Cheng, L., Wolff, J.A., et al. (1996). Efficient and sustained transgene expression in mature rat oligodendrocytes in primary culture. J. Neurosci. Res. 43, 32–41.

    Article  PubMed  CAS  Google Scholar 

  22. Sun, W.H., Burkholder, J.K., Sun, J., Culp, J., Turner, J., Lu, X.G., et al. (1995). In vivo cytokine gene transfer by gene gun reduces tumor growth in mice. Proc. Natl. Acad. Sci. USA 92, 2889–2893.

    Article  PubMed  CAS  Google Scholar 

  23. Mahvi, D.M., Burkholder, J.K., Turner, J., Culp, J., Malter, J.S., Sondel, P.M., et al. (1996). Particle-mediated gene transfer of granulocyte-macrophage colony-stimulating factor cDNA to tumor cells: Implications for a clinically relevant tumor vaccine. Hum. Gene Ther. 7, 1535–1543.

    Article  PubMed  CAS  Google Scholar 

  24. Tan, J., Yang, N.S., Turner, J.G., Niu, G.L., Maassab, H.F., Sun, J., et al. (1999). Interleukin-12 cDNA skin transfection potentiates human papillomavirus E6 DNA vaccine-induced antitumor immune response. Cancer Gene Ther. 6, 331–339.

    Article  PubMed  CAS  Google Scholar 

  25. Woffendin, C., Yang, Z., Udaykumar, K., Xu, L., Yang, N., Sheehy, M.J., et al. (1994). Non viral and viral delivery of a Human immunodeficiency virus protective gene into primary human T cells. Proc. Natl. Acad. Sci. USA 91, 11581–11585.

    Article  PubMed  CAS  Google Scholar 

  26. Hogge, G.S., Burkholder, J.K., Culp, J., Albertini, M.R., Dubielzig, R.R., Yang, N.S., et al. (1999). Preclinical development of human granulocyte-macrophage colony-stimulating factor-transfected melanoma cell vaccine using established canine cell lines and normal dogs. Cancer Gene Ther. 6, 26–36.

    Article  PubMed  CAS  Google Scholar 

  27. Staniforth, V., Chiu, L.T., and Yang, N.S. (2006) Caffeic acid suppresses UVB radiation-induced expression of interleukin-10 and activation of mitogen-activated protein kinases in mouse. Carcinogenesis 27, 1803–1811.

    Article  PubMed  CAS  Google Scholar 

  28. Chiu, S.C. and Yang, N.S. (2007) Inhibition of tumor necrosis factor-alpha through selective blockade of Pre-mRNA splicing by shikonin. Mol. Pharmacol. 71, 1640–1645.

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by a grant (No. 96-2320-B-001–008) from the National Science Council and a grant (No. 95N-1003) from the National Science and Technology Program for Agricultural Biotechnology, Taiwan.

Author information

Authors and Affiliations

  1. Agricultural Biotechnology Research Center, Academia Sinica, Nankang, Taipei 115, Taiwan, Republic of China

    Ning Sun Yang

Authors
  1. Kandan Aravindaram
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ning Sun Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ning Sun Yang .

Editor information

Editors and Affiliations

  1. Molecular Medicine (MDC), Max Delbrück Center for, Robert-Rössle-Str. 10, Berlin, 13125, Germany

    Wolfgang Walther

  2. Molecular Medicine (MDC), Max Delbrück Center for, Robert-Rössle-Str. 10, Berlin, 13125, Germany

    Ulrike S. Stein

Rights and permissions

Reprints and permissions

Copyright information

© 2009 Humana Press, a part of Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Aravindaram, K., Yang, N.S. (2009). Gene Gun Delivery Systems for Cancer Vaccine Approaches. In: Walther, W., Stein, U. (eds) Gene Therapy of Cancer. Methods in Molecular Biology™, vol 542. Humana Press. https://doi.org/10.1007/978-1-59745-561-9_9

Download citation

  • DOI: https://doi.org/10.1007/978-1-59745-561-9_9

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-934115-85-5

  • Online ISBN: 978-1-59745-561-9

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation