Summary
The human immunodeficiency virus type 1 (HIV-1) predominantly infects two main cell types: T-lymphocytes and monocyte-derived cells such as macrophages, dendritic cells, and Langerhans cells. Studies in patients aimed at uncovering and understanding the molecular mechanisms for viral persistence and latency in macrophages in particular are challenging because these cells reside in tissues and can only be extracted using invasive methods. The in vitro human monocyte-derived macrophage (MDM) model provides an amenable system to study HIV–host cell interactions at the molecular level. Infection of macrophages with a recombinant M-tropic virus that contains the green fluorescent protein (GFP) gene within its genome allows the detection of cells with integrated viral DNA that are producing viral particles. The infected cells can be studied at the single-cell level using a variety of fluorescence-based technologies such as flow cytometry, conventional and confocal microscopy, and laser capture microdissection.
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Acknowledgments
The author thanks Peter Lopez and Hao Zhang for their expertise in flow sorting, Carlos A. Pardo for use of the PALM Microlaser, and Suzanne Gartner for sharing her love and knowledge of monocyte–macrophage culturing and for making these studies possible. This work was supported by grants from The Campbell Foundation (A. Brown), National Institutes of Health R01 AI057007 (S. Gartner), and K08 DA16160 (C. Pardo).
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Brown, A.M. (2009). Use of a Macrophage-Tropic GFP-Tagged Human Immunodeficiency Virus Type 1 (HIV-1) to Study Viral Reservoirs. In: Hicks, B.W. (eds) Viral Applications of Green Fluorescent Protein. Methods in Molecular Biology™, vol 515. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-559-6_11
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DOI: https://doi.org/10.1007/978-1-59745-559-6_11
Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-59745-559-6
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