Abstract
Highly diverse antibody (Fab or scFv) libraries have become vital sources to select antibodies with high affinity and novel properties. Combinatorial strategies provide efficient ways of creating antibody libraries containing a large number of individual clones. These strategies include the reassembly of naturally occurring genes encoding the heavy and light chains from either immune or nonimmune B-cell sources, or introduction of synthetic diversity to either the framework regions (FRs) or the complementarity-determining regions (CDRs) of the variable domains of antibodies. In the late 1980s, the smallest known antigen-binding fragment was identified when a murine VH repertoire was screened for binding to lysozyme. This fragment (∼15 kDa), called a “domain antibody”, or “dAb”, is approximately four times smaller than a Fab and half the size of a scFv. Here, we describe the construction of a phage-displayed VH library and an approach to introduce genetic diversity in this library, where both diverse human CDRs and synthetic CDRs are combined into a single-domain (VH) framework.
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References
Zhang, Q., Chen, G., Liu, X., and Qian, Q. (2007) Monoclonal antibodies as therapeutic agents in oncology and antibody gene therapy. Cell Res. 17, 89–99.
Ward, E. S., Güssow, D., Griffiths, A. D., Jones, P. T., and Winter, G. (1989) Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli. Nature 341, 544–546.
Muyldermans, S., Cambillau, C., and Wyns, D. (2001) Recognition of antigens by single domain antibody fragments: the superfluous luxury of paired domains. Trends Biochem. Sci. 26, 230–235.
Holt, L. J., Herring, C., Jespers, L. S., Woolven, B. P., and Tomlinson, I. M. (2003) Domain antibodies: proteins for therapy. Trends Biotechnol. 21, 484–490.
Schellekens, H. (2002) Immunogenicity of therapeutic proteins: clinical implications and future prospects. Clin. Ther. 24, 1720–1740
Söderlind, E., Strandberg, L., Jirholt, P., Kobayashi, N., Alexeiva1, V., Åberg, A. M., et al. (2000) Recombining germline-derived CDR sequences for creating diverse single framework antibody libraries. Nature Biotechnol. 18, 852–856.
Reiter, Y., Schuck, P., Boyd, L. F., and Plaksin, D. (1999) An antibody single-domain phage display library of a native heavy chain variable region: isolation of functional single-domain VH molecules with a unique interface. J. Mol. Biol. 290, 685–698.
Jirholt, P., Ohlin, M., Borrebaeck, C. A. K., and Söderlind, E. (1998) Exploiting sequence space: shuffling in vivo formed complementarity determining regions into a master framework. Gene 215, 471–476.
Acknowledgments
This project was supported by the NIH Intramural AIDS Targeted Antiviral Program (IATAP) and the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Chen, W., Zhu, Z., Xiao, X., Dimitrov, D.S. (2009). Construction of a Human Antibody Domain (VH) Library. In: Dimitrov, A. (eds) Therapeutic Antibodies. Methods in Molecular Biology™, vol 525. Humana Press. https://doi.org/10.1007/978-1-59745-554-1_4
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DOI: https://doi.org/10.1007/978-1-59745-554-1_4
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