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Scaling-Up and Production of Therapeutic Antibodies for Preclinical Studies

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Therapeutic Antibodies

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 525))

Abstract

Monoclonal antibodies are increasingly used for treatment of various diseases, mostly cancer therapy and auto-immune diseases. New chimeric, humanized, or fully-human antibodies specific to novel or already validated targets are being developed in many academic labs as well as in commercial entities. A validation of antibodies in preclinical settings is an important component of the road from discovery to clinic. Preparing sufficient amount of high-quality antibodies for preclinical studies is a challenge in some cases. This article summarizes protocols used in research laboratories, and is based on our own experience in the establishment of stable clones of CHO cells to scaling-up of tissue cultures for production of antibodies in bioreactors.

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Abbreviations

Ab:

antibody

IgG:

immunoglobulin

Fab:

antigen-binding fragment

CDR:

complementarity-determining regions

CHO:

Chinese hamster ovary

HRP:

horseradish peroxidase

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Acknowledgments

This research was supported by the Intramural research Program of the NIH, NCI, Center for Cancer Research.

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Authors and Affiliations

  1. NCI-Frederick, National Institutes of Health, Frederick, MD, USA

    Yang Feng & Dimiter S. Dimitrov

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  1. Yang Feng
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  2. Dimiter S. Dimitrov
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    © 2009 Humana Press, a part of Springer Science+Business Media, LLC

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    Feng, Y., Dimitrov, D.S. (2009). Scaling-Up and Production of Therapeutic Antibodies for Preclinical Studies. In: Dimitrov, A. (eds) Therapeutic Antibodies. Methods in Molecular Biology™, vol 525. Humana Press. https://doi.org/10.1007/978-1-59745-554-1_26

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    • DOI: https://doi.org/10.1007/978-1-59745-554-1_26

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    • Publisher Name: Humana Press

    • Print ISBN: 978-1-934115-92-3

    • Online ISBN: 978-1-59745-554-1

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