Abstract
Symplex™ is an antibody discovery technology that identifies fully human antigen-specific antibody repertoires directly from plasma cells. The technology utilizes reverse transcription and overlap extension polymerase chain reaction performed on single-cell-sorted plasma cells, whereby the heavy- and light-chain cognate pairing of the antibodies is maintained. The isolated antibodies from a plasma cell donor reflect the diversity, affinity, and selectivity of the donor antibody repertoire, making the technology an ideal tool for identifying drug leads and studying the development of human antibody repertoires.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Meijer, PJ., Nielsen, L.S., Lantto, J., Jensen, A. (2009). Human Antibody Repertoires. In: Dimitrov, A. (eds) Therapeutic Antibodies. Methods in Molecular Biology™, vol 525. Humana Press. https://doi.org/10.1007/978-1-59745-554-1_13
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DOI: https://doi.org/10.1007/978-1-59745-554-1_13
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