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Assembling DNA Barcodes

  • Protocol
Environmental Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 410))

summary

The Barcode of Life initiative represents an ambitious effort to develop an identification system for eukaryotic life based on the analysis of sequence diversity in short, standardized gene regions. Work is furthest advanced for members of the animal kingdom. In this case, a target gene region has been selected (cytochrome c oxidase I) and pilot studies have validated its effectiveness in species discovery and identification. Based on these positive results, there is now a growing effort to both gather barcode records on a large-scale for members of this kingdom and to identify target barcode regions for the other kingdoms of eukaryotes. In this chapter, we detail the protocols involved in the assembly of DNA barcode records for members of the animal kingdom, but many of these approaches are of more general application.

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Acknowledgments

We thank Alex Borisenko, Rob Dooh, Teresa Crease, Robert Hanner, Angela Holliss, Stephanie Kirk, Paula Mackie, Pia Marquart, Erin Penton, Keith Pickthorn, Cadhla Ramsden, Sujeevan Ratnasingham, Alex Smith, Janet Topan, Taika von Konigslow, Adam Yule, and Tyler Zemlak for their aid in protocol development. Grants from the Gordon and Betty Moore Foundation, NSERC, the Canada Foundation for Innovation and the Ontario Innovation Trust aided preparation of this chapter.

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Authors and Affiliations

  1. Department of Integrative Biology, Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, Canada

    Jeremy R. deWaard, Natalia V. Ivanova, Mehrdad Hajibabaei & Paul D. N. Hebert

Authors
  1. Jeremy R. deWaard
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  2. Natalia V. Ivanova
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  3. Mehrdad Hajibabaei
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  4. Paul D. N. Hebert
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Editor information

Editors and Affiliations

  1. Center for Advanced Research in Environmental Genomics (CAREG),Department of Biology, University of Ottawa, Ottawa, Canada

    C. Cristofre Martin

  2. Department of Biochemistry, St. George’s University Medical School, St. George’s, Grenada, West Indies

    C. Cristofre Martin

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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deWaard, J.R., Ivanova, N.V., Hajibabaei, M., Hebert, P.D.N. (2008). Assembling DNA Barcodes. In: Martin, C.C., Martin, C.C. (eds) Environmental Genomics. Methods in Molecular Biology, vol 410. Humana Press. https://doi.org/10.1007/978-1-59745-548-0_15

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  • DOI: https://doi.org/10.1007/978-1-59745-548-0_15

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-777-8

  • Online ISBN: 978-1-59745-548-0

  • eBook Packages: Springer Protocols

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