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Organizational Issues in Providing High-Quality Human Tissues and Clinical Information for the Support of Biomedical Research

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Cancer Gene Profiling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 576))

Summary

Superior-quality human tissues are required to support many types of biomedical research. To be useful optimally in supporting research, not only must these tissues be accurately diagnosed, but also the specific aliquots of tissue supplied to investigators must be accurately described as part of the quality control analysis of the tissue. Tissues should be collected, processed, and stored uniformly. Some tissues are provided to investigators from tissue banks for which tissues have been collected and processed according to standard operating procedures (SOPs) of the tissue bank. Other tissues provided to support research are collected and processed according to SOPs modified to meet investigator needs and requirements, i.e., prospective collection/processing. These different models of tissue collection require different goals, designs, and SOPs. The objectives of tissue repositories also vary based on the types of tissues provided (e.g., fresh tissue aliquots, fixed paraffin-embedded tissue, paraffin tissue sections, etc.) and how the tissues are to be used in research. For example, the potential use of tissues affects the need for extensive annotation of the specimen including both clinical information (e.g., clinical outcomes) and demographics. Specifically, if the tissues are to be used for extraction of proteins or basic studies of disease processes, less clinical information, if any, may be needed than if the tissues are to be used for the correlation of an aspect of the disease process with clinical outcome or response to a specific therapy. In this review, we describe, based on our experience, the major issues that should be addressed in designing and establishing a tissue repository.

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Abbreviations

CHTN:

Cooperative Human Tissue Network

DCIS:

Ductal carcinoma in situ

DMSO:

Dimethyl sulfoxide

GMP:

Good manufacturing practices

HIPAA:

Health Insurance Portability and Accountability Act

ISBER:

International Society for Biological and Environmental Repositories

ISO:

International Organization for Standardization

LCIS:

Lobular carcinoma in situ

LN2 :

Liquid nitrogen

NCI:

National Cancer Institute

OCT:

Optimal cutting temperature (compound for embedding specimens prior to cryosectioning)

PHI:

Protected health care information

PSA:

Prostatic-specific antigen

QA:

Quality assurance/management

QC:

Quality control

SOP:

Standard operating procedure

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Acknowledgments

Our work is supported in part by the Cooperative Human Tissue Network (NCI #3U01CA044968), the Breast SPORE at UAB (NCI #1P50CA89019), the Pancreatic SPORE at UAB (NCI #P50CA101955), the Early Detection Research Network (NCI #5U24CA086359), and the Cardiovascular Medical Research Education Fund (TP1GR).

Author information

Authors and Affiliations

  1. Department of Pathology, University of Alabama at Birmingham, Birmingham, AL, USA

    Walter C. Bell

  2. Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, USA

    Katherine C. Sexton

  3. Department of Pathology, University of Alabama at Birmingham,Zeigler Research Building, Birmingham, AL, USA

    William E. Grizzle

Authors
  1. Walter C. Bell
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  2. Katherine C. Sexton
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  3. William E. Grizzle
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Editor information

Editors and Affiliations

  1. Medizinische Fak. Carl Gustav Carus, TU Dresden, Fetscherstr. 74, Dresden, 01307, Germany

    Robert Grützmann

  2. Klinik und Poliklinik für, TU Dresden, Fetscherstraße 74, Dresden, 01307, Germany

    Christian Pilarsky

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Bell, W.C., Sexton, K.C., Grizzle, W.E. (2009). Organizational Issues in Providing High-Quality Human Tissues and Clinical Information for the Support of Biomedical Research. In: Grützmann, R., Pilarsky, C. (eds) Cancer Gene Profiling. Methods in Molecular Biology, vol 576. Humana Press. https://doi.org/10.1007/978-1-59745-545-9_1

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  • DOI: https://doi.org/10.1007/978-1-59745-545-9_1

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-934115-76-3

  • Online ISBN: 978-1-59745-545-9

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