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Expression, Purification, and Crystallization of Toll/Interleukin-1 Receptor (TIR) Domains

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Toll-Like Receptors

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 517))

Summary

Toll-like receptors (TLRs) and interleukin-1 receptor (IL-1R) play crucial roles in host innate immune response against microbial infections. These receptors share a conserved cytoplasmic domain, the Toll/interleukin-1 receptor (TIR) domain, which is required for signaling through these receptors. Structural information on the TIR domains will be essential for understanding the molecular basis for signal transduction by these receptors.

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Acknowledgments

We thank Yingwu Xu and Javed Khan for their contributions to the TIR domain project. This research was supported in part by a grant from the National Institutes of Health (AI49475 to LT).

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Authors and Affiliations

  1. Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, New York, USA

    Xiao Tao

  2. Department of Biological Sciences, Columbia University, New York, USA

    Liang Tong

Authors
  1. Xiao Tao
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  2. Liang Tong
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Corresponding author

Correspondence to Liang Tong .

Editor information

Editors and Affiliations

  1. School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland

    Claire E. McCoy  & Luke A. J. O’Neill  & 

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© 2009 Humana Press, a part of Springer Science + Business Media, LLC

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Tao, X., Tong, L. (2009). Expression, Purification, and Crystallization of Toll/Interleukin-1 Receptor (TIR) Domains. In: McCoy, C.E., O’Neill, L.A.J. (eds) Toll-Like Receptors. Methods in Molecular Biology™, vol 517. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-541-1_6

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  • DOI: https://doi.org/10.1007/978-1-59745-541-1_6

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-934115-72-5

  • Online ISBN: 978-1-59745-541-1

  • eBook Packages: Springer Protocols

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