Abstract
DNA double-strand breaks (DSBs) initiate meiotic recombination in eukaryotes. We describe two strategies that use microarrays to determine the genome-wide distribution of meiotic DSBs in the yeast Saccharomyces cerevisiae. The first is a chromatin immunoprecipitation (ChIP) approach that targets the Spo11 protein, which remains covalently attached to DSB ends in certain mutant backgrounds. The second approach involves BND cellulose enrichment of the single-strand DNA (ssDNA) recombination intermediate formed by end-resection at DSB sites following Spo11 removal.
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Acknowledgments
We thank Jennifer Gerton, Hugh Cam and Shiv Grewal for technical advice, Dhruba Chattoraj and Yikang Rong for advice regarding the manuscript, and David Kaback and Jennifer Fung for communicating data in advance of publication.
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Buhler, C., Shroff, R., Lichten, M. (2009). Genome-Wide Mapping of Meiotic DNA Double-Strand Breaks in Saccharomyces cerevisiae . In: Keeney, S. (eds) Meiosis. Methods in Molecular Biology, vol 557. Humana Press. https://doi.org/10.1007/978-1-59745-527-5_10
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DOI: https://doi.org/10.1007/978-1-59745-527-5_10
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