Abstract
The flow of information in cells requires the constant remodeling of cell signaling and trafficking networks. To observe the remodeling events associated with activation of receptors on the cell surface, the authors have generated and analyzed high-resolution topographical maps of colloidal gold nanoprobes (3–10 nm) marking receptors, signaling proteins, and lipids in native membranes. The technology involves sandwiching of cells between glass cover slips and electron microscopy (EM) grids, followed by ripping. Membrane sheets on EM grids are fixed, labeled with functionalized nanoprobes, and imaged by transmission electron microscopy. Probe coordinates are extracted from digitized images and the distributions of the probes are analyzed with respect to each other and to membrane features like clathrin-coated pits, caveolae, and the cortical cytoskeleton.
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© 2007 Humana Press Inc., Totowa, NJ
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Wilson, B.S. et al. (2007). Exploring Membrane Domains Using Native Membrane Sheets and Transmission Electron Microscopy. In: McIntosh, T.J. (eds) Lipid Rafts. Methods in Molecular Biology, vol 398. Humana Press. https://doi.org/10.1007/978-1-59745-513-8_17
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DOI: https://doi.org/10.1007/978-1-59745-513-8_17
Publisher Name: Humana Press
Print ISBN: 978-1-58829-729-7
Online ISBN: 978-1-59745-513-8
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