Abstract
NF-κB/Rel transcription factors are critical regulators of immunity, inflammation, development, and cell survival. Activation of NF-κB inhibits programmed cell death (PCD) triggered by tumor necrosis factor α (TNFα) and several other stimuli. The prosurvival activity of NF-κB is also crucial to lymphopoiesis, neuroprotection, tumorigenesis, and cancer chemoresistance. The characterization of the downstream targets that mediate the prosurvival activity of NF-κB is therefore a topic of intense investigation. Early screens aimed at identifying these genes were mainly based on expression criteria and so were poised to only isolate genes already known to have protective effects. Here, we describe a new method for the identification of these genes, whereby expression libraries are screened for their ability to halt PCD in NF-κB-deficient cells. This complementation approach provides substantial advantages over other approaches, as it enables functional assessment of isolated genes without any preconceived notion about their sequence or presumed role. Expression libraries are generated from cells that are resistant to TNFα-induced cytotoxicity and are then enriched in prosurvival genes upon selection with TNFα in NF-κB/RelA-null cells, which are highly susceptible instead to this cytotoxicity. Upon enrichment, libraries are screened through a randomized two-step approach, whereby cDNAs are first tested for cytoprotective function and then for differential expression in NF-κB-proficient and NF-κB-deficient cells.
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Kuntzen, C. et al. (2007). A Method for Isolating Prosurvival Targets of NF-κB/Rel Transcription Factors. In: Borsello, T. (eds) Neuroprotection Methods and Protocols. Methods in Molecular Biology, vol 399. Humana Press. https://doi.org/10.1007/978-1-59745-504-6_8
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DOI: https://doi.org/10.1007/978-1-59745-504-6_8
Publisher Name: Humana Press
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Online ISBN: 978-1-59745-504-6
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