Abstract
The widespread occurrence of methicillin-resistant Staphylococcus aureus (MRSA) or oxacillin-resistant MRSA is a major cause of concern worldwide. Although mainly located in hospital environments, these microorganisms have been reported to have the capacity to cause infections in the community. Resistance to methicillin implies resistance to all β-lactam antibiotics and, furthermore, MRSA isolates normally harbor resistance to other families of antibiotics such as co-trimoxazole and aminoglycosides. Prompt and accurate detection of MRSA isolates is therefore extremely important in clinical microbiology laboratories. In this chapter, we review the most common methods of susceptibility testing for MRSA.
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References
Chambers, H. F. (1997) Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications. Clin. Microbiol. Rev. 10, 781–791.
Swenson, J. M., Williams, P., Killgore, G., et al. (2001) Performance of eight methods, including two new methods for detection of oxacillin resistance in a challenge set of Staphylococcus aureus organisms. J. Clin. Microbiol. 39, 3785–3788.
Van Leeuwen, W. B., Van Pelt, C., Luijendijk, A., et al. (1999) Rapid detection of methicillin resistance in Staphylococcus aureus isolates by the MRSA-screen latex agglutination test. J. Clin. Microbiol. 37, 3029, 3030.
Louie, L., Majury, A., Goodfellow, J., et al. (2001) Evaluation of a latex agglutination test (MRSA-Screen) for detection of oxacillin resistance in coagulase-negative Staphylococci. J. Clin. Microbiol. 39, 4149–4151.
National Committee for Clinical Laboratory Standards. (2003) Methods for dilution Antimicrobial Susceptibility tests for bacteria that grow aerobically. Document M7-A6 and Document M100-S13 Wayne, PA.
Skov, R., Smyth, R., Clausen, M., et al. (2003) Evaluation of cefoxitin 30 µg disc on Iso-Sensitest agar for detection of methicillin-resistant Staphylococcus aureus. J. Antimicrob. Chemother. 52, 204–207.
Felten, A., Grandry, B., Lagrange, P. H., et al. (2002) Evaluation of three techniques for detection of low-level methicillin-resistant S. aureus (MRSA): a disc diffusion method with cefoxitin and moxalactam, the Vitek 2 system, and the MRSA-screen latex agglutination test. J. Clin. Microbiol. 40, 2766–2771.
Cauwelier, B., Gordts, B., Descheemaecker, P., et al. (2004) Evaluation of a disk diffusion method with cefoxitin (30 µg) for detection of methicillin-resistant Staphylococcus aureus. Eur. J. Clin. Microbiol. Infect. Dis. 23, 389–392.
Kluytmans, J., Van Griethuysen, A., Willemse, P., et al. (2002) Performance of CHROM agar selective medium and oxacillin resistance screening agar base for identifying Staphylococcus aureus and detecting methicillin resistance. J. Clin. Microbiol. 40, 2480–2482.
Louie, L., Matsumura, S. O., Choi, E., et al. (2000) Evaluation of three rapid methods for detection of methicillin resistance in S. aureus. J. Clin. Microbiol. 38, 2170–2173.
CLSI. (2005) Performance standards for antimicrobial susceptibility testing. CLSI approved standard M100-S15. Clinical and Laboratory Standards Institute, Wayne, PA.
Pottumarthy, S., Fritsche, T. R., and Jones R. N. (2005) Evaluation of alternative disk diffusion methods for detecting mecA-mediated oxacillin resistance in an international collection of staphylococci: validation report from the SENTRY Antimicrobial Surveillance Program. Diag. Microbiol. Infect. Dis. 51, 57–62.
Velasco, D., Tomas, M., Cartelle, M., et al. (2005) Evaluation of different methods for detecting methicillin (oxacillin) resistance in Staphylococcus aureus. J. Antimicrob. Chemother. 55, 379–382.
Swenson, J. M., Tenover, F. C., and the cefoxitin disk study group. (2005) Results of disk diffusion testing with cefoxitin correlate with presence of mecA in Staphylococcus spp. J. Clin. Microbiol. 43, 3818–3823.
Smyth, R. W. and Kahlmeter G. (2005) Mannitol salt agar-cefoxitin combination as a screening medium for methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 43, 3797–3799.
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© 2007 Humana Press Inc., Totowa, NJ
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Bou, G. (2007). Minimum Inhibitory Concentration (MIC) Analysis and Susceptibility Testing of MRSA. In: Ji, Y. (eds) Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols. Methods in Molecular Biology, vol 391. Humana Press. https://doi.org/10.1007/978-1-59745-468-1_3
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DOI: https://doi.org/10.1007/978-1-59745-468-1_3
Publisher Name: Humana Press
Print ISBN: 978-1-58829-655-9
Online ISBN: 978-1-59745-468-1
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