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Cytokine Multiplex Analysis

  • Protocol
Inflammation and Cancer

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 511))

Summary

The ability to monitor gene expression in experimental and clinical samples is an essential element of modern molecular biology and cell biology research. However with the advent of a systems biology approach toward understanding cell and cancer biology, analysis of expression of a single gene is no longer desirable. Today, multiplex analysis, where the expression of 8–100 genes can be monitored in one sample, has become a routine aspect of gene expression analysis. In this chapter the various assays systems commercially available for multiplex analysis of both RNA and protein will be discussed.

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Acknowledgments

I wish to thank Claudia Jursik (Bender MedSystems), Jeanne Gaylor (BD-Pharmingen), Bruce Seligmann (HighThroughput Genomics), Aiguo Zhang (Panomics), Pankaj Oberoi (MesoS-cale Discovery), Mark Coffey (Luminex), George Quellhorst (SABiosciences) and Linda Lavigne (Thermo Fischer Scientific) for the information described in this chapter.

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Authors and Affiliations

  1. Laboratory of Experimental Immunology, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute-Frederick, Frederick, MD, USA

    Howard A. Young

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  1. Howard A. Young
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Editors and Affiliations

  1. National Cancer Institute at Frederick, SAIC-Frederick, Inc., Frederick, MD, USA

    Serguei V. Kozlov

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© 2009 Springer Science+Business Media, LLC

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Young, H.A. (2009). Cytokine Multiplex Analysis. In: Kozlov, S.V. (eds) Inflammation and Cancer. Methods in Molecular Biology™, vol 511. Humana Press. https://doi.org/10.1007/978-1-59745-447-6_4

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  • DOI: https://doi.org/10.1007/978-1-59745-447-6_4

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-934115-14-5

  • Online ISBN: 978-1-59745-447-6

  • eBook Packages: Springer Protocols

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