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Transient Transformation of Plants

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Plant Genomics

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 513))

Summary

Transient expression in plants is a valuable tool for many aspects of functional genomics and promoter testing. It can be used both to over-express and to silence candidate genes. It is also scaleable and provides a viable alternative to microbial fermentation and animal cell culture for the production of recombinant proteins. It does not depend on chromosomal integration of heterologous DNA so is a relatively facile procedure and can lead to high levels of transgene expression. Recombinant DNA can be introduced into plant cells via physical methods, via Agrobacterium or via viral vectors.

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Acknowledgements

Rothamsted receives grant-aided support from the Biotechnological and Biological Sciences Research Council, UK. We acknowledge other members of the Rothamsted Cereal Transformation Group, past and present, for their significant contribution to the protocols described here.

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  1. CPI Division, Rothamsted Research, Harpenden, Hertfordshire, UK

    Huw D. Jones

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  1. Huw D. Jones
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  2. Angela Doherty
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  3. Caroline A. Sparks
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    © 2009 Humana Press, a part of Springer Science+Business Media, LLC

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    Jones, H., Doherty, A., Sparks, C. (2009). Transient Transformation of Plants. In: Gustafson, J., Langridge, P., Somers, D. (eds) Plant Genomics. Methods in Molecular Biology™, vol 513. Humana Press. https://doi.org/10.1007/978-1-59745-427-8_8

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    • DOI: https://doi.org/10.1007/978-1-59745-427-8_8

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    • Print ISBN: 978-1-58829-997-0

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