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Murine Antigen-Induced Arthritis

  • Protocol
Arthritis Research

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 136))

Abstract

Antigen induced arthritis is a unilateral T-cell driven model caused by direct injection of an antigen into the knee joint of a FCA preimmunized animal. The chronicity is determined by antigen retention in avascular structures of the joint through charge mediated binding or antibody mediated trapping. Cationicity of the antigen is a prerequisite in this model in the mouse and commercial mBSA is a suitable antigen. Cartilage erosive character is strongly enhanced in the presence of marked antibody titer. Concomitant boosting of the immune response with Bordetella pertussis adds to this. T-cell mediated flares can be induced by local or systemic rechallenge with low dose antigen, and display a strong erosive phenotype.

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Author information

Authors and Affiliations

  1. Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands

    Wim B. van den Berg

  2. Rheumatology Research and Advanced Therapeutics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands

    Leo A. B. Joosten & Peter L. E. M. van Lent

Authors
  1. Wim B. van den Berg
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  2. Leo A. B. Joosten
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  3. Peter L. E. M. van Lent
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Editor information

Editors and Affiliations

  1. The Kennedy Institute of Rheumatology, Imperial College London, London, UK

    Andrew P. Cope

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© 2007 Humana Press Inc., Totowa, NJ

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van den Berg, W.B., Joosten, L.A.B., van Lent, P.L.E.M. (2007). Murine Antigen-Induced Arthritis. In: Cope, A.P. (eds) Arthritis Research. Methods in Molecular Medicine, vol 136. Humana Press. https://doi.org/10.1007/978-1-59745-402-5_18

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  • DOI: https://doi.org/10.1007/978-1-59745-402-5_18

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-918-5

  • Online ISBN: 978-1-59745-402-5

  • eBook Packages: Springer Protocols

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