Abstract
The Halogen assay is a new technique for measuring airborne allergen. The assay is unique in that it is capable of analyzing allergens and particles together, combining the advantages of morphological approaches and immunoassay. The Halogen assay allows direct observation of the particles that carry the allergen as well as being capable of identifying all the allergen sources an individual is exposed and sensitized to. The assay is sensitive because the extracted allergen is bound to the membrane at a high local concentration within the minute area around each particle and so is easily detected by immunostaining. It is therefore easy to detect few pollen grains.
The Halogen method supersedes other methods commonly used to identify allergens as it is capable of identifying airborne particles that are allergen sources.
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References
Tovey, E. (1995) Patent US 08/7934887, Detection of Molecules Associated with Airborne Particles. University of Sydney, Assignee.
Spieksma, F. T. M., Kramps, J. A., Plomp, A., and Koerten, K. (1991) Grass-pollen allergen carried by the smaller micronic aerosol fraction. Grana 30, 98–101.
Hinds, W. (1982) Aerosol Technology. Wiley, New York.
Razmovski, V., O’Meara, T., Hjelmroos, M., et al. (1998) Adhesive tapes as capture surfaces in Burkard sampling. Grana 37, 305–310.
O’Meara, T., DeLucca, S., Sporik, R., et al. (1998) Detection of inhaled cat allergen. Lancet 351, 1488–1489.
De Lucca, S., Taylor, D., O’Meara, T., et al. (1999) Measurement and characterisation of cockroach allergens detected during normal domestic activity. J. Allergy Clin. Immunol. 104, 672–680.
Renstrom, A., Karlsson, A. S., Manninen, A., and Tovey, E. (1999) Measuring inhaled occupational allergens: a comparison between nasal sampling and conventional air sampling on filters using pumps. Eur. Respir. J. 14(30), 538s.
Razmovski, V., O’Meara, T., and Tovey, E. (2000) A new method for simultaneous immuno-detection and morphologic identification of individual sources of pollen allergens. J. Allergy Clin. Immunol. 105, 725–731.
Tovey, E., De Lucca, S. D., Pavlicek, P., et al. (2000) The morphology of particles carrying mite, dog, cockroach and cat aeroallergens affects their efficiency of collection by nasal samplers and cascade impactors. J. Allergy Clin. Immunol. 105, S228, Abstract 676.
Lovborg, U., Baker, P., Taylor, D. J. M., et al. (1999) Subtribe-specific monoclonal antibodies to Lolium perenne. Clin. Exp. Allergy 29, 973–981.
Kirschfink, M. and Terness, P. (1998) Immunoblotting. In Molecular Diagnosis of Infectious Disease (Reischl, U. ed.), Humana, Totowa, NJ, pp. 361–371.
Poulos, L., O’Meara, T., Sporik, R., and Tovey, E. (1999) Detection of inhaled Der p 1. Clin. Exp. Allergy 29, 1232–1238.
De Lucca, S., Sporik, R., O’Meara, T., and Tovey, E. (1999) Mite Allergen (Der p 1) is not only carried on mite faeces J. Allergy Clin. Immunol. 103, 174–175.
Lovborg, U., Baker, P., and Tovey, E. (1998) A species-specific monoclonal antibody to Cynodon dactylon. Int. Arch. Allergy Immunol. 117, 220–223.
Sercombe, J. K., Pavlicek, K., Xavier, M. L., et al. (1998) (Abstract 337) Assessment of nasal and pocket air samplers. J. Allergy Clin. Immunol. 101, S80.
Graham, J., Pavlicek, J., Sercombe, M., and Xavier, M., et al. (2000) The nasal sampler. A device for sampling inhaled aeroallergens. Ann. Allergy, Asthma and Immunol. 84, 599–604.
Bjerrum, O. and Heegard, H. (ed.) (1988) Handbook of Immunoblotting of Proteins, CRC, Baco Raton, FL.
Garfin, D. and Bers, G. (1989) Basic aspects of protein blotting. In Protein Blotting: Methodology, Research and Diagnostic Applications (Baldo, B. and Tovey, E. R. ed.), Karger, Basel.
Black, S. (1998) Diagnostic application of a multi-channel immunoblot apparatus. In Molecular Diagnosis in Infectious Disease (Reischel, U. ed.), Humana, Totowa, NJ, pp. 373–396.
Tovey, E., Johnson, M., Roche, A., et al. (1989) Cloning and sequencing of a cDNA expressing a recombinant House Dust Mite protein that binds human IgE and corresponds to an important low molecular weight allergen. J. Exp. Med. 170, 1457–1462.
Tarlton, J. and Knight, P. J. (1996) Clarification of immunoblots on polyvinylidene difluoride (PVDF) membranes for transmission densitometry. J. Immunol. Methods 191, 65–69.
Knox, R. B. (1993) Grass pollen, thunderstorms and asthma. Clin. Exp. Allergy 23, 354–359.
Benyon, F., Jones, A., and Tovey, ERT. (1998) Spores of airborne, allergenic fungi are differentiated and identified by image analysis. J. Allergy Clin. Immunol. 101, S132, Abs 550.
Jones, A., Hjelmroos-Koski, M., and Tovey, E. (1999) Image analysis can be used to identify airborne allergenic pollen. J. Allergy Clin. Immunol. 103, S188, Abs 721.
Benyon, F., Jones, A., Tovey, E., and Stone, G. (1999) Differentiation of allergenic fungal spores by image analysis, with application to aerobiological counts. Aerobiologia 15, 211–223.
Hjelmroos, M., Benyon, F., Culliver, S., et al. (1999) Airborne Allergens. Interactive Identification of Allergenic Pollen and Fungal Spores, CD-ROM, Inhalix Institute of Respiratory Medicine, Sydney.
Tovey, E. R., Chapman, M. D., and Platts-Mills, T. A. E. (1981) The distribution of house dust mite allergen in the houses of patients with asthma. Am. Rev. Respir. Dis. 124, 630–635.
Schumacher, M. J., Griffith, D., and O’Rourke, M. K. (1988) Recognition of pollen and other particulate aeroantigens by immunoblot microscopy. J. Allergy Clin. Immunol. 82, 608–616.
Suphioglu, C., Singh, M. B., Taylor, B. B., et al. (1992) Mechanism of grass pollen-induced asthma. Lancet 339, 569–572.
Acevedo, F., Vesterberg, O., and Bayard, C. (1998) Visualization and quantification of birch-pollen allergens directly on air-sampling filters. Allergy 53, 594–601.
Holmquist, L. and Vesterberg, O. (1999) Luminescence immunoassay of pollen allergens on air sampling polytetrafluoroethylene filters. J. Biochem. Biophys. Methods 41, 49–60.
Green, B. J., Sercombe, J. K., and Tovey, E. R. (2005) Fungal fragments and undocumented conidia function as new aeroallergen sources. J. Allergy Clin. Immunol. 115, 1043–1048.
Green, B. J., Millecchia, L. L., Blachere, F. M., Tovey, E. R., Beezhold, D. H., and Schmechel, D. (2006) Dual fluorescent halogen immunoassay for bioaerosols using confocal microscopy. Anal. Biochem. 354, 151–153.
Green, B. J., Schmechel, D., Sercombe, J. K., and Tovey, E. R. (2005) Enumeration and detection of aerosolized Aspergillus fumigatus and Penicillium chrysogenum conidia and hyphae using a novel double immunostaining technique. J. Immunol. Methods 307, 127–134.
Green. B. J., Schmechel, D., and Tovey, E. R. (2005) Detection of aerosolized Alternaria alternata conidia, hyphae, and fragments by using a novel double-immunostaining technique. Clin. Diagn. Lab. Immunol. 12, 1114–1116.
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Tovey, E., Lucca, S.D., Poulos, L., O’Meara, T. (2008). The Halogen Assay - A New Technique for Measuring Airborne Allergen. In: Jones, M.G., Lympany, P. (eds) Allergy Methods and Protocols. Methods in Molecular Medicine, vol 138. Humana Press. https://doi.org/10.1007/978-1-59745-366-0_19
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