Abstract
The emergence of microarray technology as a novel tool in molecular biology has led to significant progress in many biomedical disciplines, including gerontology. Both cDNA and oligonucleotide-based DNA microarrays are now widely used to identify the basic physiological mechanisms of aging and to uncover the molecular mechanisms underlying the biological effects of anti-aging drugs. Two different protocols covering both cDNA and oligonucleotide microarray platforms, with radioactive and nonradioactive (fluorescent) labeling, are detailed in the manuscript. These in-depth protocols provide a background for the technical aspects of everyday work with DNA microarrays.
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© 2007 Humana Press Inc., Totowa, NJ
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Anisimov, S.V. (2007). Application of DNA Microarray Technology to Gerontological Studies. In: Tollefsbol, T.O. (eds) Biological Aging. Methods in Molecular Biology™, vol 371. Humana Press. https://doi.org/10.1007/978-1-59745-361-5_19
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DOI: https://doi.org/10.1007/978-1-59745-361-5_19
Publisher Name: Humana Press
Print ISBN: 978-1-58829-658-0
Online ISBN: 978-1-59745-361-5
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