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Identification and Analysis of Essential Genes in Haemophilus influenzae

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Microbial Gene Essentiality: Protocols and Bioinformatics

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 416))

Abstract

The human respiratory pathogen Haemophilus influenzae, a Gram-negative bacterium, is the first freeliving organism to have its complete genome sequenced, providing the opportunity to apply genomic-scale approaches to study gene function. This chapter provides an overview of a highly efficient, in vitro mariner transposon-based method that exploits the natural transformation feature of this organism for the identification of essential genes. In addition, we describe strategies for conditional expression systems that would facilitate further analysis of this class of genes. Finally, we outline a method based on the approach used in H. influenzae for identifying essential genes that can be applied to other bacteria that are not naturally transformable.

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Author information

Authors and Affiliations

  1. Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA

    Sandy M.S. Wong & Brian J. Akerley

Authors
  1. Sandy M.S. Wong
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  2. Brian J. Akerley
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Editor information

Editors and Affiliations

  1. Burnham Institute for Medical Research, La Jolla, California

    Andrei L. Osterman

  2. Fellowship for Interpretation of Genomes (FIG), Burr Ridge, Illinois

    Svetlana Y. Gerdes

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© 2008 Humana Press Inc., a part of Springer Science+Business Media, LLC

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Wong, S.M., Akerley, B.J. (2008). Identification and Analysis of Essential Genes in Haemophilus influenzae . In: Osterman, A.L., Gerdes, S.Y. (eds) Microbial Gene Essentiality: Protocols and Bioinformatics. Methods in Molecular Biology™, vol 416. Humana Press. https://doi.org/10.1007/978-1-59745-321-9_3

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  • DOI: https://doi.org/10.1007/978-1-59745-321-9_3

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-378-7

  • Online ISBN: 978-1-59745-321-9

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