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Animal Models for Bone Tissue Engineering Purposes

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Sourcebook of Models for Biomedical Research

Abstract

To assess the efficacy of engineered tissues, it is necessary to have (1) appropriate large animal models that mimic the clinical setting and (2) relevant methods of monitoring the biofuntionality of these tissues. However, developing these tissue constructs is a step-by-step process in which numerous variables such as scaffold design, source of stem cells and mode of growth factor application have to be optimized. After an in vitro optimization phase, the use of small animal models to optimize these various parameters and sort out any teething problems is recommended before launching into large animal models. Depending on the experimental aims, engineered tissues can be transplanted into either ectopic sites (subcutaneously or intramuscularly) or orthotopic sites. In all these experimental studies, non invasive imaging methods (X-ray, magnetic resonance, in vivo fluorescence, ultrasound imaging methods, etc.) as well as detailed quantitative molecular and histological analyses have been used to monitor the in vivo behavior of the engineered constructs. In this chapter we take stock of the present state of the art in this field.

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Authors and Affiliations

  1. Unité Pédagogique de Pathologie Chirurgicale, Ecole Nationale Veterinaire d’Alfort, Maisons Alfort, France

    Véronique Viateau DMV, PhD

  2. Laboratoire de Recherches Orthopediques, Université Denis Diderot, Paris, France

    Delphine Logeart-Avramoglou PhD & Geneviève Guillemin PhD

  3. Laboratoire de Recherches Orthopediques, Faculte de Medecine Lariboisiere Saint-Louis, Universite Denis Diderot Paris 7, Paris, France

    Hervé Petite PhD

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  1. Véronique Viateau DMV, PhD
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  2. Delphine Logeart-Avramoglou PhD
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  3. Geneviève Guillemin PhD
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  4. Hervé Petite PhD
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  1. Oregon National Primate Research Center, Beaverton, OR

    P. Michael Conn PhD (Associate Director and Senior Scientist, Professor of Physiology and Pharmacology, Professor of Cell and Developmental Biology) (Associate Director and Senior Scientist, Professor of Physiology and Pharmacology, Professor of Cell and Developmental Biology)

  2. Oregon Health and Science University, Portland, OR

    P. Michael Conn PhD (Associate Director and Senior Scientist, Professor of Physiology and Pharmacology, Professor of Cell and Developmental Biology) (Associate Director and Senior Scientist, Professor of Physiology and Pharmacology, Professor of Cell and Developmental Biology)

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Viateau, V., Logeart-Avramoglou, D., Guillemin, G., Petite, H. (2008). Animal Models for Bone Tissue Engineering Purposes. In: Conn, P.M. (eds) Sourcebook of Models for Biomedical Research. Humana Press. https://doi.org/10.1007/978-1-59745-285-4_74

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  • DOI: https://doi.org/10.1007/978-1-59745-285-4_74

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