Abstract
In Drosophila, input, pacemaker, and output genes are expressed circadianly. mRNA oscillations contribute largely to these rhythms. Determining RNA levels of circadian genes is thus frequently necessary to understand their regulation, or the effect of mutations and genetic manipulations on the function of the circadian pacemaker. RNA extraction is the prelude to several techniques aimed at measuring RNA levels. The procedure presented in this chapter is a rapid method to obtain a clean preparation of total RNA from fly heads that can be used for RNase protection, Northern blots. and real-time polymerase chain reaction (see Chapters 23–25).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Dunlap, J. C. (1999) Molecular bases for circadian clocks. Cell 96, 271–290.
Zeng, H., Hardin, P. E., and Rosbash, M. (1994) Constitutive overexpression of the Drosophila period protein inhibits period mRNA cycling. EMBO J. 13, 3590–3598.
Kaneko, M., Helfrich-Forster, C., and Hall, J. C. (1997) Spatial and temporal expression of the period and timeless genes in the developing nervous system of Drosophila: newly identified pacemakers candidates and novel features of clock gene product cycling. J. Neurosci. 17, 6745–6760.
Helfrich-Forster, C. (1996) Drosophila rhythms: from brain to behavior. Semin. Cell Dev. Biol. 7, 791–802.
Kaneko, M., and Hall, J. C. (2000) Neuroanatomy of cells expressing clock genes in Drosophila: transgenic manipulation of the period and timeless genes to mark the perikarya of circadian pacemaker neurons and their projections. J. Comp. Neurol. 422, 66–94.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Humana Press Inc.
About this protocol
Cite this protocol
Emery, P. (2007). RNA Extraction From Drosophila Heads. In: Rosato, E. (eds) Circadian Rhythms. Methods in Molecular Biology™, vol 362. Humana Press. https://doi.org/10.1007/978-1-59745-257-1_20
Download citation
DOI: https://doi.org/10.1007/978-1-59745-257-1_20
Publisher Name: Humana Press
Print ISBN: 978-1-58829-417-3
Online ISBN: 978-1-59745-257-1
eBook Packages: Springer Protocols