Abstract
Quantitative proteomic measurements are of significant interest in studies aimed at discovering disease biomarkers and providing new insights into biological pathways. A quantitative cysteinyl-peptide enrichment technology (QCET) can be employed to achieve higher efficiency, greater dynamic range, and higher throughput in quantitative proteomic studies based on the use of stable isotope-labeling techniques combined with high-resolution capillary or nano-scale liquid chromatography-mass spectrometry (LC-MS) measurements. The QCET approach involves specific 16O/18O-labeling of tryptic peptides, high-efficiency enrichment of cysteinyl-peptides, and confident protein identification and quantification using high mass accuracy LC-Fourier transform ion cyclotron resonance mass spectrometry (FTICR) measurements and a previously established database of accurate mass and LC elution time information for the labeled peptides. This methodology has been initially demonstrated by using proteome profiling of naïve and in vitro-differentiated human mammary epithelial cells as an example, which initially resulted in the identification and quantification of 603 proteins in a single LC-FTICR analysis. QCET provides not only highly efficient enrichment of cysteinyl-peptides for more extensive proteome coverage and improved labeling efficiency for better quantitative measurements, but more importantly, a high-throughput strategy suitable for quantitative proteome analysis where extensive or parallel proteomic measurements are required, such as in time course studies of specific pathways and clinical sample analyses for biomarker discovery.
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Liu, T., Qian, WJ., Camp, D.G., Smith, R.D. (2007). The Use of a Quantitative Cysteinyl-Peptide Enrichment Technology for High-Throughput Quantitative Proteomics. In: Sechi, S. (eds) Quantitative Proteomics by Mass Spectrometry. Methods in Molecular Biology, vol 359. Humana Press. https://doi.org/10.1007/978-1-59745-255-7_7
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DOI: https://doi.org/10.1007/978-1-59745-255-7_7
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