Summary
Mice provide a useful model for the study of immune deficiency caused by chronic alcohol abuse. Their suitability is related to several factors, including in particular the extensive knowledge base in the immunology of mice already existing in the literature. Specific modeling of the immunodeficiency of the chronic human alcoholic requires that ethanol must be administered to the model for a significant portion of its life span. In mice, it has proven to be necessary to administer ethanol daily for up to 32 wk or longer to observe all the immune abnormalities that occur in middle-aged alcoholic humans. Such time spans are problematic with many of the common protocols for ethanol administration. It has been shown by others and confirmed by our group that the most practical way of accomplishing such long protocols is by administering ethanol in water as the only choice of water. Details of management of the chronic ethanol mouse colony are described here that are necessary for the success of such studies, including methods for initiating ethanol administration, maintenance of barrier protection, monitoring weight gain, strain differences and fetal alcohol exposure.
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References
1. MacGregor, R. R., Louria, D. B. (1997) Alcohol and infection [review]. Curr. Clin. Top. Infect. Dis. 17, 291–315.
2. Cook, R. T. (1998) Alcohol abuse, alcoholism, and damage to the immune system—a review. Alcohol Clin. Exp. Res. 22, 1927–1942.
3. Happel, K. I., and Nelson, S. (2005) Alcohol, immunosuppression, and the lung. Proc. Am. Thorac. Soc. 2, 428–432.
4. Jerrells, T., and Sibley, D. (1995) Effects of ethanol on cellular immunity to facultative intracellular bacteria. Alcohol Clin. Exp. Res. 19, 11–16.
5. Nelson, S., Summer, W., Bagby, G., et al. (1991) Granulocyte colony-stimulating factor enhances pulmonary host defenses in normal and ethanol-treated rats. J. Infect. Dis. 164, 901–906.
6. Lister, P. D., Gentry, M. J., and Preheim, L. C. (1993) Granulocyte colony stimulating factor protects control rats but not ethanol-fed rats from fatal pneumococcal pnneumonia. J. Infect. Dis. 168, 922–926.
7. Bermudez, L. E., Petrofsky, M., Kolonoski, P., and Young, L. S. (1992) An animal model of Mycobacterium avium complex disseminated infection after colonization of the intestinal tract. J. Infect. Dis. 165, 75–79.
8. Mendenhall, C. L., Grossman, C. J., and Roselle, G. A. (1990) Host response to Mycobacterium infection in the alcoholic rat. Gastroenterology 99, 1723–1726.
9. Saad, A. J., Domiati-Saad, R., and Jerrells, T. R. (1993) Ethanol ingestion increases susceptibility of mice to Listeria monocytogenes. Alcohol Clin. Exp. Res. 17, 75–85.
10. Messingham, K. A., Faunce, D. E., and Kovacs, E. J. (2002) Alcohol, injury, and cellular immunity. Alcohol 28, 137–149.
11. Kakihana, R., and Moore, J. A. (1976) Circadian rhythm of corticosterone in mice: The effect of chronic consumption of alcohol. Psychopharmacologia 46, 301–305.
12. Sipp, T. L., Blank, S. E., Lee, E. G., and Meadows, G. G. (1993) Plasma corticosterone response to chronic ethanol consumption and exercise stress. Proc. Soc. Exp. Biol. Med. 204, 184–190.
13. Reeb-Whitaker, C. K., Paigen, B., Beamer, W. G., et al. (2001) The impact of reduced frequency of cage changes on the health of mice housed in ventilated cages. Lab. Animals 35, 58–73.
14. Song, K., Coleman, R. A., Zhu, X., et al. (2002) Chronic ethanol consumption by mice results in activated splenic T cells. J. Leuk. Biol. 72, 1109–1116.
15. Cook, R. T., Schluetter, A. J., Coleman, R. A., et al. (2007) Thymocytes, pre-B cells and organ changes in a mouse model of chronic ethanol ingestion. Absence of subset specific glucoeorticoid-induced immune cell loss. Alcohol Clin. Exp. Res. 31, 1746–1758.
Acknowledgements
Work in the authors’ laboratory has been supported by grant numbers AA09598 and AA014405 from the NIH, and the Department of Pathology of the University of Iowa.
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Coleman, R.A., Young, B.M., Turner, L.E., Cook, R.T. (2008). A Practical Method of Chronic Ethanol Administration in Mice. In: Nagy, L.E. (eds) Alcohol. Methods in Molecular Biology™, vol 447. Humana Press. https://doi.org/10.1007/978-1-59745-242-7_4
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DOI: https://doi.org/10.1007/978-1-59745-242-7_4
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