Abstract
The search for rapid and reproducible in vivo angiogenesis and antiangiogenesis assays is an area of intense interest. These types of assays are extremely useful in testing putative drugs and biological agents and for the comparison and enhancement of in vitro tests. The Matrigel plug assay is one such assay and has proved to be a relatively quick and easy method to evaluate both angiogenic and antiangiogenic compounds in vivo. Initial indications of the levels of activity of strong angiogenic or antiangiogenic compounds can be visually assessed even as the plugs come out of the mouse because there are color differences in the plugs compared to the controls. Further quantitation is then needed to determine levels of angiogenic/antiangiogenic activity, and this can be performed using a variety of methods. This chapter presents an overview of the basic methods used to set up both angiogenic and antiangiogenic assays, discusses factors influencing variability, and discusses the methods for quantitating the plugs obtained. The Matrigel plug assay provides another useful tool in angiogenesis research.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Kleinman, H. K., McGarvy, M. L., Liotta, L. A., Gehron-Robbey, P., Tryggvasson, K., Martin, G. R. (1982) Isolation and characterization of type IV procollagen, laminin, and heparan sulfate proteoglycan from the EHS sarcoma. Biochemistry 24, 6188–6193.
Kubota, Y., Kleinman, H. K., Martin, G. R., Lawley, T. J. (1988) Role of laminin and basement membrane in the morphological differentiation of human endothelial cells into capillary-like structures. J Cell Biol 107, 1589–1598.
Grant, D. S., Kinsella, J. L., Fridman, R., (1992) Interaction of endothelial cells with a laminin A chain peptide (SIKVAV) in vitro and induction of angiogenic behavior in vivo. J Cell Physiol 153, 614–625.
Passaniti, A., Taylor, R. M., Pili, R., (1992) A simple, quantitative method for assessing angiogenesis and antiangiogenic agents using reconstituted basement membrane, heparin, and fibroblast growth factor. Lab Invest 67, 519–528.
Kibbey, M. C., Corcoran, M. L., Wahl, L. M., Kleinman, H. K. (1994) Laminin SIKVAV peptide-induced angiogenesis in vivo is potentiated by neutrophils. J Cell Phys 160, 185–193.
Auerbach, R., Lewis, R., Shinners, B., Kubai, L., Akhtar N. (2003) Angiogenesis assays: a critical overview. Clin Chem 49, 32–40.
Johns, A., Freay, A. D., Fraser, W., Korach, K. S., Rubanyi, G. M. (1996) Disruption of estrogen receptor gene prevents 17 β estradiol-induced angiogenesis in transgenic mice. Endocrinology 137, 4511–4513.
Prewett, M., Huber, J., Li, Y., (1999) Antivascular endothelial growth factor receptor (fetal liver kinase 1) monoclonal antibody inhibits tumor angiogenesis and growth of several mouse and human tumors. Cancer Res 59, 5208–5218.
Babaei, S., Teichert-Kuliszewska, K., Zhang, Q., Jones, N., Dumont, D. J., Stewart, D. J. (2003) Angiogenic actions of angiopoietin-1 require endothelium-derived nitric oxide. Am J Pathol 1927–1936.
Akhtar, N., Dickerson, E. B., Auerbach, R. (2002) The sponge/Matrigel angiogenesis assay. Angiogenesis 5, 75–80.
Malinda, K. M., Goldstein, A. L., Kleinman, H. K. (1997) Thymosin β4 stimulates directional migration of human umbilical vein endothelial cells. FASEB J 11, 474–481.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Malinda, K.M. (2009). In Vivo Matrigel Migration and Angiogenesis Assay. In: Murray, C., Martin, S. (eds) Angiogenesis Protocols. Methods in Molecular Biology, vol 467. Humana Press. https://doi.org/10.1007/978-1-59745-241-0_17
Download citation
DOI: https://doi.org/10.1007/978-1-59745-241-0_17
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-58829-907-9
Online ISBN: 978-1-59745-241-0
eBook Packages: Springer Protocols